Virus Purification

Based on our unique shelled bead technology, BioToolomics develops tailored resins for efficient and cost-effective purification of viral vectors, virus-like particles and various exosomes used in cell and gene therapy and vaccine industries.

ViralPolish™ chromatography media specifically for purification of viruses and other nano-structures such as exosomes and nucleic acids etc. It is a class of dual layer polymer beads possessing an inert external shell with tightly controlled pore size and internal polyfunctional ligands for rapid high capacity binding of impurities.

Large particles, such as viruses, are excluded from the beads. The beads can be packed in a column where viruses will pass through the column bed and collected in flow through fraction, whilst impurities will be captured within the beads. This affords a very gentle purification process resulting in efficient clean up and high recovery of active virus particles.

ViralPolish is available in a range of outer shells (i.e. different pore sizes), and two different impurity binding chemistries (see below).

The media is stable in most of the chemical conditions experienced in bioprocessing industry. Regulatory support file is available to support large scale manufacturing use.

ViralPolish™ 5000A; ViralPolish™ 5000B – Good for small viruses such as small size AAVs etc. The pore size on the shell is similar or slightly smaller than that of Capto core 400.

ViralPolish™ 6000A; ViralPolish™ 6000B and ViralPolish™ 7000A; ViralPolish™ 7000B – Good for larger AAVs, medium to large size viruses and VLPs, exosomes, DNA clearance etc. The pore size on the shell of 7000 range is similar to that of Capto core 700. The pore size of 6000 range sits between 5000 range and 7000 range.

ViralPolish™ 8000A; ViralPolish™ 8000B and ViralPolish™ 9000A; ViralPolish™ 9000B and ViralPolish™ 10000A; ViralPolish™ 10000B – Good for even larger size viruses and VLPs, exosomes, DNA (>1kb) clearance etc, where impure species are very big.

Key features of “A”: Mixed-mode anion ligand with mild hydrophobicity; can be easily re-generated and re-used; but its binding capacity may be compromised.

Key features of “B”: Mixed-mode anion ligand with strong hydrophobicity; have higher loading capacity but is less easy to re-generate.

SepFast DUO IEX is unique ion-exchange chromatography media utilising dual functionalities. Individual beads are coated with an inert polymer out-layer giving a size-exclusion effect. Inside the bead are anion-exchange or cation-exchange ligands. This type of novel media is designed for the selective purification of target molecules based on both molecular weights and charges.

The media is stable in most of the chemical conditions experienced in bioprocessing industry. Regulatory support file is available to support large scale manufacturing use.

SepFast™ DUO 5000 Q – Good for small viruses such as small size AAVs etc. The pore size on the shell is similar or slightly smaller than that of Capto core 400.

SepFast™ DUO 6000 Q and SepFast™ DUO 7000 Q – Good for larger AAVs, medium to large size viruses and VLPs, exosomes, DNA clearance etc. The pore size on the shell of 7000 range is similar to that of Capto core 700. The pore size of 6000 range sits between 5000 range and 7000 range.

SepFast™ DUO 8000 Q, SepFast™ DUO 9000 Q, and SepFast™ DUO 10000 Q – Good for even larger size viruses and VLPs, exosomes, DNA (>1kb) clearance etc, where impure species are very big.

Gel filtration (or called size exclusion chromatography, SEC) is a well-proven versatile technique, which is widely used for separating viruses / exosomes based on their sizes (molecular weights).

SepFast 4B; SepFast 6B – For purifying very large molecules (e.g. plasmids, viruses etc) under gravity or very low pressure.

SepFast CL-2B; SepFast CL-4B; SepFast CL-6B – For purifying very large molecules (e.g. exosomes, plasmids, viruses etc) under gravity or low pressure.

SepFast 4 High Flow; SepFast 6 High Flow – For purifying very large molecules (e.g. plasmids, viruses etc) at process scale under pressure.

Virus Purification Products