Ion Exchange (IEX) media

IEX SepFast HighRes Plus

£160.72£2,036.43 ex. VAT

Description

Q (DEAE, S, CM) SepFast™ HighRes Plus is a group of ion-exchange chromatography media with reduced bead size (20 – 50 μm). They are specially designed for high resolution and high binding capacity purification of biological molecules, where impure components are hard to separate by normal bioprocessing chromatography media.

The core advantages are:

  • High binding capacity
  • High resolution
  • High sample loading capacity at high flow
  • High productivity

IEX SepFast HighRes Plus is made of a composite of agarose and dextran to give increased dynamic binding capacities. The base matrix is highly cross-linked. The media is very stable to most of the chemical conditions experienced in the bioprocessing industry.

Pre-packed columns for screening and process development use are available at following sizes: 1 ml, 5 ml, 3.8 ml (7x100mm ID x BH), 9.5 ml (11x100mm ID x BH), 20 ml (16x100mm ID x BH), 53 ml (26x100mm ID x BH). All these columns have connections fully compatible to Akta systems. Column hardware has 6 bar (0.6 MPa) pressure rating. See Column Instruction for details of HiSep and Optio column hardware. 

Pre-packed columns for pilot to production scale use with column diameter ranging from 5cm to 45cm are available on request. For bulk loose media, please contact us.

The range of IEX SepFast HighRes Plus media is developed and supported for production scale chromatography use. Regulatory Support File (RSF) is available to assist process validation and submissions to regulatory authorities.

More information about IEX SepFast HighRes Plus.

Characteristics of IEX SepFast HighRes Plus

Matrix

Highly cross-linked agarose grafted with dextran chain

Functional Group

Quaternary ammonium strong anion (Q SepFast HighRes Plus)

Diethylaminoethyl weak anion (DEAE SepFast HighRes Plus)

Sulfopropyl strong cation (SP SepFast HighRes Plus)

Carboxymethyl weak cation (CM SepFast HighRes Plus)

Total Ionic Capacity

0.1-0.2 mmol/ml (Q SepFast HighRes Plus)

0.08-0.13 mmol/ml (DEAE SepFast HighRes Plus)

0.1-0.2 mmol/ml (SP SepFast HighRes Plus)

0.08-0.13 mmol/ml (CM SepFast HighRes Plus)

Dynamic Binding Capacity*

>130 mg BSA / ml (Q SepFast HighRes Plus)

>130 mg BSA / ml (DEAE SepFast HighRes Plus)

>130 mg lysozyme / ml (SP SepFast HighRes Plus)

>130 mg lysozyme / ml (CM SepFast HighRes Plus)

*Test condition: 10 mM buffer pH 7.5, 5 mg/ml protein to load, residence time 5 min, 50% breakthrough

Particle Size

20 – 50 μm

Pressure-Flow Property

30 -150 cm/hr is recommended

Operational Pressure

Up to 3 bar

pH Stability

2-14 (short term) and 3-12 (long term) (Q SepFast HighRes Plus and DEAE SepFast HighRes Plus)

2-14 (short term) and 4-12 (long term) (SP SepFast HighRes Plus and CM SepFast HighRes Plus)

Working Temperature

+4°C – +30°C

Chemical Stability

All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol

Avoid

Oxidizing agents, anionic detergents (Q SepFast HighRes Plus and DEAE SepFast HighRes Plus)

Oxidizing agents, cationic detergents (SP SepFast HighRes Plus and CM SepFast HighRes Plus)

Storage

20% ethanol (Q SepFast HighRes Plus)

20% ethanol (DEAE SepFast HighRes Plus)

20% ethanol + 0.2 M sodium acetate (SP SepFast HighRes Plus)

20% ethanol (CM SepFast HighRes Plus)