£236.19 – £919.60 ex. VAT
SepFast 4HF and 6HF are highly cross-linked plain agarose beads often used for fractionation of large biological molecules based on their molecular weights. Gel Filtration is a proven technique, which is widely used for size-based molecular separation. The media is also often used as a good base matrix to immobilise affinity ligands.
| Matrix | 4HF: 4% agarose beads (highly cross-linked) 6HF: 6% agarose beads (highly cross-linked) |
|---|---|
| Particle Size | 50 – 150 µm |
| Molecules to Separate | 4HF: Up to 4 x 10⁷ Dalton 6HF: Up to 5 x 10⁶ Dalton |
| Operating Flow Velocity | Normally 10 to 300 cm/hour |
| pH Stability | 3-13 (short term) and 4-11 (long term) |
| Working Temperature | 4°C – 30°C |
| Chemical Stability | All commonly used buffers; 20% ethanol |
| Avoid | Oxidizing agents |
| Storage | The media should be stored in 20% ethanol to prevent microbial growth. |