Epoxy-activated SepFast MAG

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Introduction


Epoxy-activated SepFast MAG is a magnetic agarose resin that can be readily employed to make various magnetic affinity chromatography adsorbents for both small scale and large scale purification applications. Epoxy activated SepFast is useful in coupling both small molecules and very large molecules. After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.

The SepFast MAG base matrix has been highly cross-linked. It shows high mechanical rigidity and allows harsher operational conditions, such as concentrated organic solvents, to be applied.

Epoxy-activated agarose can be used for the immobilization of sugars and other carbohydrates via stable ether linkages with hydroxyl groups. It can also be used for the coupling of primary amine-containing or thiol containing molecules. Coupling biospecific ligands to Epoxy-activated agarose is a successful and well-documented technique. The reaction is very easy to conduct. No toxic chemicals or special equipment is required. All the chemical bonds formed are very stable.

There is a choice of two different Epoxy activated magnetic resins that suit various ligands and applications.

  • Epoxy activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material, useful for coupling protein molecules. 
  • Epoxy activated SepFast MAG 6HF is made of highly cross-linked 6% agarose containing magnetic material. It has a high level of epoxy groups for applications that require high ligand densities, useful for coupling small ligands.

Coupling manual is supplied with each product.

Characteristics of Epoxy-activated SepFast MAG

 
Group to be coupled -NH2, -OH, -SH
Coupling conditions pH 9 – 13, 20oC to 40oC, 16-48 h, organic solvents possible
Matrix SepFast MAG 4HF: Highly cross-linked 4% magnetic agarose
SepFast MAG 6HF: Highly cross-linked 6% magnetic agarose
Particle size* 50 – 150 μm
Activation level >30 μmol epoxy / ml for SepFast MAG 4HF
>80 μmol epoxy / ml for SepFast MAG 6HF
pH stability 4 -13 (ligand dependent)
Chemical stability Stable to all commonly used aqueous chemicals, provided the ligand to be coupled can withstand.Certain organic solvents could be used.
Storage 4oC – 8oC

*Other particle size available on request.

Ordering information

 
Product Quantity Code no. Price GBP
Epoxy activated SepFast MAG 4HF 5 ml 410201-5ML 74.20
  50 ml 410201-50ML POR
  1 litre 410201-1L POR
Epoxy activated SepFast MAG 6HF 5 ml 410204-5ML 74.20
  50 ml 410204-50ML POR
  1 litre 410204-1L POR

How to order

Operation Manual

Operation Manual