NHS-activated SepFast MAG

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Introduction


The base matrix is activated through a long hydrophilic spacer arm with N-hydroxysuccinimide at the end that is particularly helpful in the immobilization of structurally restricted ligands such as proteins and peptides. It reacts directly with the primary amine groups in molecules to be immobilized to form stable amide bond.

NHS (N-hydroxysuccinimide)-activated SepFast MAG is used to make magnetic adsorbents coupled with molecules containing amine groups. Coupling biospecific ligands to NHS-activated agarose is a successful and well-documented technique. The coupling reaction is spontaneous, rapid and easy to carry out. No toxic chemicals or special equipment is required.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer propertie to various molecules.

NHS activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It shows high mechanical rigidity and allows harsher operational conditions.

This pre-activated resin can be readily employed to make various magnetic affinity chromatography adsorbents for a variety of purification applications. After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet.

Coupling manual is supplied with each product.

Characteristics of NHS-activated SepFast MAG media

 
Group to be coupled -NH2
Coupling conditions pH 6 – 9, 4oC to 30oC, 2 - 16 h, organic solvents possible
Matrix SepFast MAG 4HF: highly cross-linked 4% magnetic agarose
Particle size* 50 – 150 μm
Activation level 5 - 19 μmol active group / ml medium
pH stability 4 - 13 (ligand dependent)
Chemical stability Stable to all commonly used aqueous chemicals, provided the ligand to be coupled can withstand
Storage 4oC – 8oC

*Other particle size available on request.

Ordering information

 
Product Quantity Code no. Price GBP
NHS-activated SepFast MAG 4HF 5 ml 330201-5ML 119.19
  50 ml 330201-50ML POR
  1 Litre 330201-1L POR

How to order

Operation Manual

Operation Manual