Hydrophobic Interaction Chromatography (HIC) Media

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Introduction


SepFast Butyl, SepFast Pentyl, SepFast Hexyl, SepFast Phenyl, SepFast Heptyl and SepFast Octyl are hydrophobic interaction chromatography (HIC) adsorbents having a carbon chain of C4, C5, C6, C6 ring, C7 and C8, respectively. They are specially designed for the purification of biological molecules based on their hydrophobicity profiles.

HIC is a versatile technique and could show high selectivity to individual molecules according to their exposed hydrophobic zones. It is particularly useful for intermediate and final-stage purifications. A HIC medium normally binds at moderate to high salt concentrations. It is logical to place HIC step after an IEX step where molecules are usually eluted at high salt conditions.

HIC media shows much milder purification conditions than reversed phase chromatography (RPC) media. Better biological activity could be maintained in HIC operations than RPC operations.

SepFast HIC media offers a broad range of choice from C4 to C8. Generally speaking, the longer the carbon chain, the higher the surface hydrophobicity. Researchers have the chance to choose the best HIC medium for their applications. The core advantages are:

  • High sample loading capacity
  • More choices than other suppliers
  • High separation power

The base matrix is made of agarose that has been highly cross-linked. It is very stable to most of the chemical conditions experienced in the bioprocessing industry.

For each type of HIC ligand, there is a choice of 3 different base matrices according to the pore accessibility of target molecules. The feature and selection guide is listed as follows:

400 serial 500 serial 600 serial
SepFast Butyl
-400		 SepFast Butyl
-500		 SepFast Butyl
-600
SepFast Pentyl
-400		 SepFast Pentyl
-500		 SepFast Pentyl
-600
SepFast Hexyl
-400		 SepFast Hexyl
-500		 SepFast Hexyl
-600
SepFast Phenyl
-400		 SepFast Phenyl
-500		 SepFast Phenyl
-600
SepFast Heptyl
-400		 SepFast Heptyl
-500		 SepFast Heptyl
-600
SepFast Phenyl
-400		 SepFast Octyl
-500		 SepFast Octyl
-600
The above HIC media is designed to purify peptides or small proteins The above HIC media is designed to purify most medium to large proteins. The above HIC media is designed to purify large to very large proteins.

Characteristics of SepFast HIC Media

 
Butyl-400
Butyl-500
Butyl-600		 Pentyl-400
Pentyl-500
Pentyl-600		 Hexyl-400
Hexyl-500
Hexyl-600		 Phenyl-400
Phenyl-500
Phenyl-600		 Heptyl-400
Heptyl-500
Heptyl-600		 Octyl-400
Octyl-500
Octyl-600
Matrix Highly cross-linked polysaccharide
Functional
group		 -(CH2)3
CH3		 -(CH2)4
CH3		 -(CH2)5
CH3
-C6H6		 -(CH2)6
CH3		 -(CH2)7
CH3
Particle
size		 50 – 150 μm
Pressure
-flow
property*		 >1000 cm/h for 400 serials; >500 cm/h for 500 serials; >300 cm/h for 600 serials
Operational
pressure		 Up to 3 bar
pH
stability		 2-14 (short term) and 3-12 (long term)
Working
temperature		 4oC – 30oC
Chemical
stability		 All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% acetonitrile, 30% isopropanol, 70% ethanol, 3 M (NH4)2SO4
Storage 20% ethanol

*Measured in a 32 mm ID column at a bed height of 20 cm.

Ordering information

 
SepFast Pentyl Products SepFast Hexyl Products SepFast Phenyl Products SepFast Heptyl Products SepFast Octyl Products
Butyl Products
Description Quantity Code no. Price GBP
SepFast Butyl-400 25 ml 480101 £82.00
  100 ml 480102 £216.00
  1 Litre 480103 POR
Disposable SepFast Butyl-400 column 5 x 1 ml 480104 £124.00
  1 x 5 ml 480105 £89.00
  1 x 10 ml 480106 £134.00
  1 x 20 ml 480107 £215.00
SepFast Butyl-500 25 ml 480201 £82.00
  100 ml 480202 £216.00
  1 Litre 480203 POR
Disposable SepFast Butyl-500 column 5 x 1 ml 480204 £124.00
  1 x 5 ml 480205 £89.00
  1 x 10 ml 480206 £134.00
  1 x 20 ml 480207 £215.00
SepFast Butyl-600 25 ml 480301 £82.00
  100 ml 480302 £216.00
  1 Litre 480303 POR
Disposable SepFast Butyl-600 column 5 x 1 ml 480304 £124.00
  1 x 5 ml 480305 £89.00
  1 x 10 ml 480306 £134.00
  1 x 20 ml 480307 £215.00
SepFast Butyl Products SepFast Hexyl Products SepFast Phenyl Products SepFast Heptyl Products SepFast Octyl Products
Pentyl Products
Description Quantity Code no. Price GBP
SepFast Pentyl-400 25 ml 480401 £82.00
  100 ml 480402 £216.00
  1 Litre 480403 POR
Disposable SepFast Pentyl-400 column 5 x 1 ml 480404 £124.00
  1 x 5 ml 480405 £89.00
  1 x 10 ml 480406 £134.00
  1 x 20 ml 480407 £215.00
SepFast Pentyl-500 25 ml 480501 £82.00
  100 ml 480502 £216.00
  1 Litre 480503 POR
Disposable SepFast Pentyl-500 column 5 x 1 ml 480504 £124.00
  1 x 5 ml 480505 £89.00
  1 x 10 ml 480506 £134.00
  1 x 20 ml 480507 £215.00
SepFast Pentyl-600 25 ml 480601 £82.00
  100 ml 480602 £216.00
  1 Litre 480603 POR
Disposable SepFast Pentyl-600 column 5 x 1 ml 480604 £124.00
  1 x 5 ml 480605 £89.00
  1 x 10 ml 480606 £134.00
  1 x 20 ml 480607 £215.00
SepFast Butyl Products SepFast Pentyl Products SepFast Phenyl Products SepFast Heptyl Products SepFast Octyl Products
Hexyl Products
Description Quantity Code no. Price GBP
SepFast Hexyl-400 25 ml 480701 £82.00
  100 ml 480702 £216.00
  1 Litre 480703 POR
Disposable SepFast Hexyl-400 column 5 x 1 ml 480704 £124.00
  1 x 5 ml 480705 £89.00
  1 x 10 ml 480706 £134.00
  1 x 20 ml 480707 £215.00
SepFast Hexyl-500 25 ml 480801 £82.00
  100 ml 480802 £216.00
  1 Litre 480803 POR
Disposable SepFast Hexyl-500 column 5 x 1 ml 480804 £124.00
  1 x 5 ml 480805 £89.00
  1 x 10 ml 480806 £134.00
  1 x 20 ml 480807 £215.00
SepFast Hexyl-600 25 ml 480901 £82.00
  100 ml 480902 £216.00
  1 Litre 480903 POR
Disposable SepFast Hexyl-600 column 5 x 1 ml 480904 £124.00
  1 x 5 ml 480905 £89.00
  1 x 10 ml 480906 £134.00
  1 x 20 ml 480907 £215.00
SepFast Butyl Products SepFast Pentyl Products SepFast Hexyl Products SepFast Heptyl Products SepFast Octyl Products
Phenyl Products
Description Quantity Code no. Price GBP
SepFast Phenyl-400 25 ml 481001 £82.00
  100 ml 481002 £216.00
  1 Litre 481003 POR
Disposable SepFast Phenyl-400 column 5 x 1 ml 481004 £124.00
  1 x 5 ml 481005 £89.00
  1 x 10 ml 481006 £134.00
  1 x 20 ml 481007 £215.00
SepFast Phenyl-500 25 ml 481101 £82.00
  100 ml 481102 £216.00
  1 Litre 481103 POR
Disposable SepFast Phenyl-500 column 5 x 1 ml 481104 £124.00
  1 x 5 ml 481105 £89.00
  1 x 10 ml 481106 £134.00
  1 x 20 ml 481107 £215.00
SepFast Phenyl-600 25 ml 481201 £82.00
  100 ml 481202 £216.00
  1 Litre 481203 POR
Disposable SepFast Phenyl-600 column 5 x 1 ml 481204 £124.00
  1 x 5 ml 481205 £89.00
  1 x 10 ml 481206 £134.00
  1 x 20 ml 481207 £215.00
SepFast Butyl Products SepFast Pentyl Products SepFast Hexyl Products SepFast Phenyl Products SepFast Octyl Products
HeptylProducts
Description Quantity Code no. Price GBP
SepFast Heptyl-400 25 ml 481301 £82.00
  100 ml 481302 £216.00
  1 Litre 481303 POR
Disposable SepFast Heptyl-400 column 5 x 1 ml 481304 £124.00
  1 x 5 ml 481305 £89.00
  1 x 10 ml 481306 £134.00
  1 x 20 ml 481307 £215.00
SepFast Heptyl-500 25 ml 481401 £82.00
  100 ml 481402 £216.00
  1 Litre 481403 POR
Disposable SepFast Heptyl-500 column 5 x 1 ml 481404 £124.00
  1 x 5 ml 481405 £89.00
  1 x 10 ml 481406 £134.00
  1 x 20 ml 481407 £215.00
SepFast Heptyl-600 25 ml 481501 £82.00
  100 ml 481502 £216.00
  1 Litre 481503 POR
Disposable SepFast Heptyl-600 column 5 x 1 ml 481504 £124.00
  1 x 5 ml 481505 £89.00
  1 x 10 ml 481506 £134.00
  1 x 20 ml 481507 £215.00
SepFast Butyl Products SepFast Pentyl Products SepFast Hexyl Products SepFast Phenyl Products SepFast Heptyl Products
Octyl Products
Description Quantity Code no. Price GBP
SepFast Octyl-400 25 ml 481601 £82.00
  100 ml 481602 £216.00
  1 Litre 481603 POR
Disposable SepFast Octyl-400 column 5 x 1 ml 481604 £124.00
  1 x 5 ml 481605 £89.00
  1 x 10 ml 481606 £134.00
  1 x 20 ml 481607 £215.00
SepFast Octyl-500 25 ml 481701 £82.00
  100 ml 481702 £216.00
  1 Litre 481703 POR
Disposable SepFast Octyl-500 column 5 x 1 ml 481704 £124.00
  1 x 5 ml 481705 £89.00
  1 x 10 ml 481706 £134.00
  1 x 20 ml 481707 £215.00
SepFast Octyl-600 25 ml 481801 £82.00
  100 ml 481802 £216.00
  1 Litre 481803 POR
Disposable SepFast Octyl-600 column 5 x 1 ml 481804 £124.00
  1 x 5 ml 481805 £89.00
  1 x 10 ml 481806 £134.00
  1 x 20 ml 481807 £215.00


>>Click here for SepFast Butyl Products

>>Click here for SepFast Pentyl Products

>>Click here for SepFast Hexyl Products

>>Click here for SepFast Phenyl Products

>>Click here for SepFast Octyl Products

>>Click here for SepFast Octyl Products

How to order

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Data Sheet

Data sheet