Carboxyl activated SepFast MAG
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Introduction
Carboxyl-activated SepFast MAG is used to make magnetic chromatography adsorbents.
The base matrix is activated by attaching a long 10 atom hydrophilic chain with a carboxyl group at the end. This reacts with amine containing ligands in the presence of carbodiimide agent to form a chemically stable amide linkage. The coupling chemistry is a successful and well-documented technique.
Carboxyl-activated SepFast MAG 4 High Flow (4HF) is made of highly cross-linked
4% agarose with magnetic properties.
Agarose has long been used for chromatographic separations due to its excellent
hydrophilic and low non-specific-binding nature. The particles have an open pore
structure with excellent mass transfer properties to large protein molecules.
The pre-activated base matrix can be readily employed to make various magnetic chromatography adsorbents for a variety of purification applications. After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet.
Coupling manual is supplied with each product.
Characteristics of Carboxyl-activated SepFast MAG 4HF
Group to be coupled | -NH2 |
Coupling conditions | pH 4.5 – 7.5, 4oC to 30oC, 3-16 h, organic solvents possible |
Matrix | Highly cross-linked 4% magnetic agarose |
Particle size* | 50 – 150 μm |
Activation level | 8-19 μmol Carboxyl / ml medium |
pH stability | 4-13 (ligand dependent) |
Chemical stability | Stable to all commonly used aqueous chemicals, provided the ligand to be coupled can withstand |
Storage | 4oC – 8oC |
*Other particle size available on request.
Ordering information
Product | Quantity | Code no. | Price GBP |
Carboxyl-activated SepFast MAG 4HF | 5 ml | 380201-5ML | £75.41 |
50 ml | 380201-50ML | POR | |
1 Litre | 380201-1L | POR |