Carboxyl activated SepFast MAG
|>>Introduction||>>Product Charactistics||>>Ordering Info||>>How to Order|
Carboxyl-activated SepFast MAG is used to make magnetic chromatography adsorbents.
The base matrix is activated by attaching a long 10 atom hydrophilic chain with a carboxyl group at the end. This reacts with amine containing ligands in the presence of carbodiimide agent to form a chemically stable amide linkage. The coupling chemistry is a successful and well-documented technique.
Carboxyl-activated SepFast MAG 4 High Flow (4HF) is made of highly cross-linked
4% agarose with magnetic properties.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules.
The pre-activated base matrix can be readily employed to make various magnetic chromatography adsorbents for a variety of purification applications. After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet.
Coupling manual is supplied with each product.
Characteristics of Carboxyl-activated SepFast MAG 4HF
|Group to be coupled||-NH2|
|Coupling conditions||pH 4.5 – 7.5, 4oC to 30oC, 3-16 h, organic solvents possible|
|Matrix||Highly cross-linked 4% magnetic agarose|
|Particle size*||50 – 150 μm|
|Activation level||8-19 μmol Carboxyl / ml medium|
|pH stability||4-13 (ligand dependent)|
|Chemical stability||Stable to all commonly used aqueous chemicals, provided the ligand to be coupled can withstand|
|Storage||4oC – 8oC|
*Other particle size available on request.
|Product||Quantity||Code no.||Price GBP|
|Carboxyl-activated SepFast MAG 4HF||5 ml||380201-5ML||£75.41|