Amine activated SepFast MAG

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Introduction


Amine-activated SepFast MAG is used to make magnetic chromatography adsorbents.

The base matrix is activated by attaching a long hydrophilic chain with an amine group at the end. This reacts with either carboxyl containing ligands in the presence of carbodiimide agent, or aldehyde containing ligands in the presence of reducing agent. The coupling chemistry is a successful and well-documented technique.

Amine-activated SepFast MAG 4 High Flow (4HF) is made of highly cross-linked 4% agarose with magnetic properties.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules.

The pre-activated base matrix can be readily employed to make various magnetic chromatography adsorbents for a variety of purification applications. After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet.

Coupling manual is supplied with each product.

Characteristics of Amine-activated SepFast MAG 4HF

 
Group to be coupled -COOH, CHO
Coupling conditions pH 4.5 – 7.5, 4oC to 30oC, 3-16 h, organic solvents possible
Matrix Highly cross-linked 4% agarose beads
Particle size* 50 – 150 μm
Activation level 10 -25 μmol amine / ml medium 
pH stability 4 -13 (ligand dependent)
Chemical stability Stable to all commonly used aqueous chemicals, provided the ligand to be coupled can withstand
Storage 4oC – 8oC

*Other particle size available on request.

Ordering information

 
Product Quantity Code no. Price GBP
Amine-activated SepFast MAG 4HF 5 ml 350201-5ML 75.41
  50 ml 350201-50ML POR
  1 Litre 350201-1L POR

How to order

Operation Manual

Operation Manual