Aldehyde-activated SepFast MAG

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Introduction


The base matrix is activated through oxidation to generate aldehyde groups. It reacts directly with the primary amine groups in molecules to be immobilized in the presence of reducing agents to form chemically stable secondary amine linkages.

Aldehyde-activated SepFast MAG is a magnetic resin for the preparation of custom magnetic affinity chromatography media. Coupling biospecific ligands to aldehyde-activated agarose is a successful and well-documented technique. 

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.

The SepFast MAG base matrix has been highly cross-linked. It shows high mechanical rigidity, allowing harsher operational conditions.

This pre-activated agarose base matrix can be readily employed to make various magnetic affinity chromatography adsorbents for a variety of purification applications. After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet.

There is a choice of 2 different base matrices that suit various molecules to be coupled or purified.

  • Aldehyde-activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It has large pore size and is suitable for coupling or purifying large molecules. 
  • Aldehyde-activated SepFast MAG 6HF is made of highly cross-linked 6% agarose containing magnetic material. It has moderate pore size and is suitable for coupling smaller molecules.

Coupling manual is supplied with each product.

Characteristics of Aldehyde-activated SepFast MAG

 
Group to be coupled -NH2
Coupling conditions pH 4 – 10, 4oC to 30oC, 3-16 h, organic solvents possible
Matrix SepFast MAG 4HF: Highly cross-linked 4% magnetic agarose
SepFast MAG 6HF: Highly cross-linked 6% magnetic agarose
Particle size* 50 – 150 μm
Activation level > 5 μmol aldehyde / ml medium
pH stability 4 -13 (ligand dependent)
Chemical stability Stable to all commonly used aqueous chemicals, provided the ligand to be coupled can withstand
Storage 4oC – 8oC

*Other particle size available on request.

Ordering information

 
Product Quantity Code no. Price GBP
Aldehyde-activated SepFast MAG 4HF 5 ml 340201-5ML 62.08
  50 ml 340201-50ML POR
  1 Litre 340201-1L POR
Aldehyde-activated SepFast MAG 6HF 5 ml 340204-5ML 62.08
  50 ml 340204-50ML POR
  1 Litre 340204-1L POR

How to order

Operation Manual

Operation Manual