Epoxy-activated agarose beads can be used for the immobilization of sugars and other carbohydrates via stable ether linkages with hydroxyl groups. It can also be used for the coupling of primary amine-containing or thiol-containing molecules.

Coupling biospecific ligands to Epoxy-activated agarose is a successful and well-documented technique. The reaction is very easy to conduct. No toxic chemicals or special equipment is required. All the chemical bonds formed are very stable.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.
The SepFast base matrix has been highly cross-linked. It shows high mechanical rigidity, allowing high flow throughput with reduced back pressure.

Epoxy activated SepFast is useful in coupling both small molecules and very large molecules. This pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography media for both small scale and large scale purification applications.

There is a choice of two different Epoxy activated base matrices that suit various ligands and applications.

• Epoxy activated SepFast 4HF is made of highly cross-linked 4% beaded agarose, useful for coupling protein ligands.
• Epoxy activated SepFast 6HF is made of highly cross-linked 6% beaded agarose. It has a high level of epoxy groups for applications that require high ligand densities, useful for coupling small molecules or peptides etc.

Coupling manual is supplied with each product.