Epoxy-activated SepFast

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Introduction


Epoxy-activated agarose beads can be used for the immobilization of sugars and other carbohydrates via stable ether linkages with hydroxyl groups. It can also be used for the coupling of primary amine-containing or thiol-containing molecules.

Coupling biospecific ligands to Epoxy-activated agarose is a successful and well-documented technique. The reaction is very easy to conduct. No toxic chemicals or special equipment is required. All the chemical bonds formed are very stable.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.
The SepFast base matrix has been highly cross-linked. It shows high mechanical rigidity, allowing high flow throughput with reduced back pressure.

Epoxy activated SepFast is useful in coupling both small molecules and very large molecules. This pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography media for both small scale and large scale purification applications.

There is a choice of two different Epoxy activated base matrices that suit various ligands and applications.

  • Epoxy activated SepFast 4HF is made of highly cross-linked 4% beaded agarose, useful for coupling protein ligands.
  • Epoxy activated SepFast 6HF is made of highly cross-linked 6% beaded agarose. It has a high level of epoxy groups for applications that require high ligand densities, useful for coupling small molecules or peptides etc.

Coupling manual is supplied with each product.

Characteristics of Epoxy-activated SepFast

 
Group to be coupled -NH2, -OH, -SH
Coupling conditions pH 9 – 13, 20oC to 40oC, 16-48 h, organic solvents possible
Matrix SepFast 4HF: Highly cross-linked 4% agarose beads
SepFast 6HF: Highly cross-linked 6% agarose beads
Particle size 50 – 150 μm
Activation level >30 μmol epoxy / ml for SepFast 4HF
>80 μmol epoxy / ml for SepFast 6HF
pH stability 3 -13 (ligand dependent)
Chemical stability Stable to all commonly used aqueous chemicals, provided the ligand to be coupled can withstand. Certain organic solvents could be used.
Storage 4oC – 8oC

Ordering information

 
Product Quantity Code no. Price GBP
Epoxy activated SepFast 4HF 50 ml 410101-50ML 221.60
  300 ml 410101-300ML POR
  1 litre 410101-1L POR
Epoxy activated SepFast 6HF 50 ml 410104-50ML 221.60
  300 ml 410104-300ML POR
  1 litre 410104-1L POR

How to order

Operation Manual

Operation Manual