Aldehyde-activated SepFast

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The base matrix is activated through oxidation to generate aldehyde groups. It reacts directly with the primary amine groups in molecules to be immobilized in the presence of reducing agents to form chemically stable secondary amine linkages.

Aldehyde-activated agarose beads have a well-proven track record for the preparation and use of custom affinity chromatography media. Coupling biospecific ligands to aldehyde-activated agarose is a successful and well-documented technique.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.

The SepFast base matrix has been highly cross-linked. It shows high mechanical rigidity, allowing high flow throughput with reduced back pressure.

This pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography media for both small scale and large scale purification applications.

There is a choice of 2 different base matrices that suit various molecules to be coupled or purified.

  • Aldehyde-activated SepFast 4 High Flow (4HF) is made of highly cross-linked 4% beaded agarose. It has large pore size and is suitable for coupling or purifying large molecules.
  • Aldehyde-activated SepFast 6 High Flow (6HF) is made of highly cross-linked 6% beaded agarose. It has moderate pore size and is suitable for coupling smaller molecules.

Coupling manual is supplied with each product.

Characteristics of Aldehyde-activated SepFast 4HF / 6HF

Group to be coupled -NH2
Coupling conditions pH 4 – 10, 4oC to 30oC, 3-16 h, organic solvents possible
Matrix SepFast 4HF: Highly cross-linked 4% agarose beads
SepFast 6HF: Highly cross-linked 6% agarose beads
Particle size 50 – 150 μm
Activation level > 5 μmol aldehyde / ml medium
pH stability 3 -13 (ligand dependent)
Chemical stability Stable to all commonly used aqueous chemicals, provided the ligand to be coupled can withstand
Storage 4oC – 8oC

Ordering information

Product Quantity Code no. Price GBP
Aldehyde-activated SepFast 4HF 5 ml 340101-5ML 42.56
  50 ml 340101-50ML POR
  1 Litre 340101-1L POR
Aldehyde-activated SepFast 6HF 5 ml 340104-5ML 42.56
  50 ml 340104-50ML POR
  1 Litre 340104-1L POR

How to order

Operation Manual

Operation Manual