The base matrix is activated through oxidation to generate aldehyde groups. It reacts directly with the primary amine groups in molecules to be immobilized in the presence of reducing agents to form chemically stable secondary amine linkages.

Aldehyde-activated agarose beads have a well-proven track record for the preparation and use of custom affinity chromatography media. Coupling biospecific ligands to aldehyde-activated agarose is a successful and well-documented technique.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.

The SepFast base matrix has been highly cross-linked. It shows high mechanical rigidity, allowing high flow throughput with reduced back pressure.

This pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography media for both small scale and large scale purification applications.

There is a choice of 2 different base matrices that suit various molecules to be coupled or purified.

• Aldehyde-activated SepFast 4 High Flow (4HF) is made of highly cross-linked 4% beaded agarose. It has large pore size and is suitable for coupling or purifying large molecules.
• Aldehyde-activated SepFast 6 High Flow (6HF) is made of highly cross-linked 6% beaded agarose. It has moderate pore size and is suitable for coupling smaller molecules.

Coupling manual is supplied with each product.