NHS-activated SepFast

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The base matrix is activated through a long hydrophilic spacer arm with N-hydroxysuccinimide at the end that is particularly helpful in the immobilization of structurally restricted ligands such as proteins and peptides. It reacts directly with the primary amine groups in molecules to be immobilized to form stable amide bonds.

NHS (N-hydroxysuccinimide)-activated agarose has a well-proven track record for the preparation and use of custom affinity chromatography media. Coupling biospecific ligands to NHS-activated agarose is a successful and well-documented technique. The coupling reaction is spontaneous, rapid and easy to carry out. No toxic chemicals or special equipment is required.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.

NHS activated SepFast 4HF is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure in a packed column.

NHS-activated SepFast media forms a chemically stable amide bond with ligands containing primary amine groups. This pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography adsorbents for both small scale and large scale purification applications.

Coupling manual is supplied with each product.

Characteristics of NHS-activated SepFast media

Group to be coupled -NH2
Coupling conditions pH 6 – 9, 4oC to 30oC, 2 - 16 h, organic solvents possible
Matrix SepFast 4HF: highly cross-linked 4% agarose
Particle size 50 – 150 μm
Activation level 5 - 19 μmol active group / ml medium
pH stability 3 -13 (ligand dependent)
Chemical stability Stable to all commonly used aqueous chemicals, provided the ligand to be coupled can withstand
Storage 4oC – 8oC

Ordering information

Product Quantity Code no. Price GBP
NHS-activated SepFast 4HF 5 ml 330101-5ML 66.93
  50 ml 330101-50ML POR
  1 Litre 330101-1L POR

How to order

Operation Manual

Operation Manual