CNBr-activated SepFast

>>Introduction >>Product Charactistics >>Ordering Info >>How to Order


The base matrix is reacted with cyanogen bromide (CNBr) to create active groups that react with the primary amine groups in the molecules to be immobilized.

CNBr-activated agarose beads have a well-proven track record for the preparation and use of custom affinity chromatography media. Coupling biospecific ligands to CNBr-activated agarose is a widely used, successful, and well-documented technique. The coupling reaction is spontaneous, rapid and easy to carry out. No toxic chemicals or special equipment is required.

CNBr activated SepFast 4 High Flow (4HF) is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particle has an open pore structure with excellent mass transfer properties to large protein molecules.

Our pre-activated SepFast beads can be readily employed to make various affinity chromatography media for both small scale and large scale purification applications. The application areas cover immobilization of proteins, peptides and nucleic acids.

Coupling manual is supplied with each product.

Characteristics of CNBr-activated SepFast 4HF

Group to be coupled -NH2
Coupling conditions pH 6 - 10, 4oC to 30oC, 3-16 h, organic solvents possible
Matrix Highly cross-linked 4% agarose beads
Particle size 50 – 150 μm
Swelling factor 3 – 5 ml / g powder
Coupling level >5 mg BSA / ml medium 
pH stability 3 -11 (ligand dependent)
Chemical stability Stable to all commonly used aqueous chemicals, provided the ligand to be coupled can withstand
Storage 4oC – 8oC

Ordering information

Product Quantity Code no. Price GBP
CNBr-activated SepFast 4HF 1 g 310101-1G 68.03
  5 g 310101-5G 163.51
  500 g 310101-500G POR
  We also provide other bulk volumes. Please contact us for further information.

How to order

Operation Manual

Operation Manual