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The base matrix is reacted with cyanogen bromide (CNBr) to create active groups that react with the primary amine groups in the molecules to be immobilized.
CNBr-activated agarose beads have a well-proven track record for the preparation and use of custom affinity chromatography media. Coupling biospecific ligands to CNBr-activated agarose is a widely used, successful, and well-documented technique. The coupling reaction is spontaneous, rapid and easy to carry out. No toxic chemicals or special equipment is required.
CNBr activated SepFast 4 High Flow (4HF) is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure.
Agarose has long been used for chromatographic
separations due to its excellent hydrophilic and low non-specific-binding
nature. The particle has an open pore structure with excellent mass transfer
properties to large protein molecules.
Our pre-activated SepFast beads can be readily employed to make various affinity chromatography media for both small scale and large scale purification applications. The application areas cover immobilization of proteins, peptides and nucleic acids.
Coupling manual is supplied with each product.
Characteristics of CNBr-activated SepFast 4HF
|Group to be coupled||-NH2|
|Coupling conditions||pH 6 - 10, 4oC to 30oC, 3-16 h, organic solvents possible|
|Matrix||Highly cross-linked 4% agarose beads|
|Particle size||50 – 150 μm|
|Swelling factor||3 – 5 ml / g powder|
|Coupling level||>5 mg BSA / ml medium|
|pH stability||3 -11 (ligand dependent)|
|Chemical stability||Stable to all commonly used aqueous chemicals, provided the ligand to be coupled can withstand|
|Storage||4oC – 8oC|
|Product||Quantity||Code no.||Price GBP|
|CNBr-activated SepFast 4HF||1 g||310101-1G||£68.03|
|We also provide other bulk volumes. Please contact us for further information.|