Amine activated SepFast

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The base matrix is activated by attaching a long hydrophilic chain with an amine group at the end. This reacts with either carboxyl containing ligands in the presence of carbodiimide agent, or aldehyde containing ligands in the presence of reducing agent. The coupling chemistry is a successful and well-documented technique.

Amine-activated SepFast 4 High Flow (4HF) is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules.

The pre-activated base matrix can be readily employed to make various custom affinity chromatography adsorbents for both small scale and large scale purification applications.

Coupling manual is supplied with each product.

Characteristics of Amine-activated SepFast 4HF

Group to be coupled -COOH, CHO
Coupling conditions pH 4.5 – 7.5, 4oC to 30oC, 3-16 h, organic solvents possible
Matrix Highly cross-linked 4% agarose beads
Particle size 50 – 150 μm
Activation level 10 -25 μmol amine / ml medium 
pH stability 3 -13 (ligand dependent)
Chemical stability Stable to all commonly used aqueous chemicals, provided the ligand to be coupled can withstand
Storage 4oC – 8oC

Ordering information

Product Quantity Code no. Price GBP
Amine-activated SepFast 4HF 5 ml 350101-5ML 51.05
  50 ml 350101-50ML POR
  1 Litre 350101-1L POR

How to order

Operation Manual

Operation Manual