SepFastTM HighRes IEX Media

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Introduction


SepFast HighRes Q, SepFast HighRes DEAE, SepFast HighRes S and SepFast HighRes CM are, respectively, strong anion, weak anion, strong cation and weak cation exchange adsorbents. They are specially designed for high resolution purification of biological molecules in which impure components are hard to separate by normal bioprocessing chromatography media.

SepFast HighRes media has a balanced design to offer high resolution power and also high loading capacity to separate similar components in bioprocessing applications. The core advantages are:

  • High resolution at high loading
  • High sample loading capacity at high flow
  • High productivity

The base matrix is made of a composite of polysaccharides that have been highly cross-linked. The media is very stable to most of the chemical conditions experienced in the bioprocessing industry.

For each type of ion exchange medium, there is a choice of three different base matrices according to pore accessibility of target molecules. The feature and selection guide is listed as follows:

400 serial 500 serial 600 serial
SepFast HighRes
Q-400		 SepFast HighRes
Q-500		 SepFast HighRes
Q-600
SepFast HighRes
DEAE-400		 SepFast HighRes
DEAE-500		 SepFast HighRes
DEAE-600
SepFast HighRes
S-400		 SepFast HighRes
S-500		 SepFast HighRes
S-600
SepFast HighRes
CM-400		 SepFast HighRes
CM-500		 SepFast HighRes
CM-600
The above media is designed to purify smaller components (e.g. M.W. <100K Dalton). The above media is designed to purify antibodies from aggregates and other impurities. The above media is designed to purify large proteins, plasmids or viral particles.

Characteristics of SepFast HighRes IEX Media

 
HighRes Q-400
HighRes Q-500
HighRes Q-600		 HighRes DEAE-400
HighRes DEAE-500
HighRes DEAE-600		 HighRes S-400
HighRes S-500
HighRes S-600		 HighRes CM-400
HighRes CM-500
HighRes CM-600
Matrix Highly cross-linked polysaccharide composites
Functional group Quaternary ammonium strong anion Diethyl-aminoethyl weak anion Sulfo strong cation Carboxy-
methyl weak cation
Total ionic capacity 0.04-0.18 mmol/ml 0.04-0.18 mmol/ml 0.04-0.18 mmol/ml 0.04-0.18 mmol/ml
Particle size 20 – 50 μm
Dynamic binding capacity* >150 mg/ml BSA for HighRes Q-400 >150 mg/ml BSA for HighRes DEAE-400 >60 mg/ml hIgG for HighRes S-500 >120 mg/ml lysozyme for HighRes CM-400
Pressure-flow property** To be run at a residence time of 2 min and above.
Operational pressure Up to 3 bar
pH stability 2-14 (short term) and 3-12 (long term)
Working temperature 4oC – 30oC
Chemical stability All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol
Total ionic capacity Oxidizing agents, anionic detergents Oxidizing agents, cationic detergents
Storage 20% ethanol 20% ethanol 20% ethanol + 0.2 M sodium acetate 20% ethanol

*Measured at a breakthrough of 10%.
**Measured in a 32 mm ID column at a bed height of 20 cm.

Ordering information

 
SepFast HighRes DEAE Products SepFast HighRes S Products SepFast HighRes CM Products
HighRes Q Products
Description Quantity Code no. Price GBP
SepFast HighRes Q-400 25 ml 470101 £89.00
  100 ml 470102 £238.00
  1 Litre 470103 POR
Disposable SepFast HighRes Q-400 column 5 x 1 ml 470104 £126.00
  1 x 5 ml 470105 £91.00
  1 x 10 ml 470106 £134.00
  1 x 20 ml 470107 £215.00
SepFast HighRes Q-500 25 ml 470201 £89.00
  100 ml 470202 £238.00
  1 Litre 470203 POR
Disposable SepFast HighRes Q-500 column 5 x 1 ml 470204 £126.00
  1 x 5 ml 470205 £91.00
  1 x 10 ml 470206 £134.00
  1 x 20 ml 470207 £215.00
SepFast HighRes Q-600 25 ml 470301 £89.00
  100 ml 470302 £238.00
  1 Litre 470303 POR
Disposable SepFast HighRes Q-600 column 5 x 1 ml 470304 £126.00
  1 x 5 ml 470305 £91.00
  1 x 10 ml 470306 £134.00
  1 x 20 ml 470307 £215.00
SepFast HighRes Q Products SepFast HighRes S Products SepFast HighRes CM Products
HighRes DEAE Products
Description Quantity Code no. Price GBP
SepFast HighRes DEAE-400 25 ml 470401 £89.00
  100 ml 470402 £238.00
  1 Litre 470403 POR
Disposable SepFast HighRes DEAE-400 column 5 x 1 ml 470404 £126.00
  1 x 5 ml 470405 £91.00
  1 x 10 ml 470406 £134.00
  1 x 20 ml 470407 £215.00
SepFast HighRes DEAE-500 25 ml 470501 £89.00
  100 ml 470502 £238.00
  1 Litre 470503 POR
Disposable SepFast HighRes DEAE-500 column 5 x 1 ml 470504 £126.00
  1 x 5 ml 470505 £91.00
  1 x 10 ml 470506 £134.00
  1 x 20 ml 470507 £215.00
SepFast HighRes DEAE-600 25 ml 470601 £89.00
  100 ml 470602 £238.00
  1 Litre 470603 POR
Disposable SepFast HighRes DEAE-600 column 5 x 1 ml 470604 £126.00
  1 x 5 ml 470605 £91.00
  1 x 10 ml 470606 £134.00
  1 x 20 ml 470607 £215.00
SepFast HighRes Q Products SepFast HighRes DEAE Products SepFast HighRes CM Products
HighRes S Products
Description Quantity Code no. Price GBP
SepFast HighRes S-400 25 ml 470701 £89.00
  100 ml 470702 £238.00
  1 Litre 470703 POR
Disposable SepFast HighRes S-400 column 5 x 1 ml 470704 £126.00
  1 x 5 ml 470705 £91.00
  1 x 10 ml 470706 £134.00
  1 x 20 ml 470707 £215.00
SepFast HighRes S-500 25 ml 470801 £89.00
  100 ml 470802 £238.00
  1 Litre 470803 POR
Disposable SepFast HighRes S-500 column 5 x 1 ml 470804 £126.00
  1 x 5 ml 470805 £91.00
  1 x 10 ml 470806 £134.00
  1 x 20 ml 470807 £215.00
SepFast HighRes S-600 25 ml 470901 £89.00
  100 ml 470902 £238.00
  1 Litre 470903 POR
Disposable SepFast HighRes S-600 column 5 x 1 ml 470904 £126.00
  1 x 5 ml 470905 £91.00
  1 x 10 ml 470906 £134.00
  1 x 20 ml 470907 £215.00
SepFast HighRes Q Products SepFast HighRes DEAE Products SepFast HighRes S Products
HighRes CM Products
Description Quantity Code no. Price GBP
SepFast HighRes CM-400 25 ml 471001 £89.00
  100 ml 471002 £238.00
  1 Litre 471003 POR
Disposable SepFast HighRes CM-400 column 5 x 1 ml 471004 £126.00
  1 x 5 ml 471005 £91.00
  1 x 10 ml 471006 £134.00
  1 x 20 ml 471007 £215.00
SepFast HighRes CM-500 25 ml 471101 £89.00
  100 ml 471102 £238.00
  1 Litre 471103 POR
Disposable SepFast HighRes CM-500 column 5 x 1 ml 471104 £126.00
  1 x 5 ml 471105 £91.00
  1 x 10 ml 471106 £134.00
  1 x 20 ml 471107 £215.00
SepFast HighRes CM-600 25 ml 471201 £89.00
  100 ml 471202 £238.00
  1 Litre 471203 POR
Disposable SepFast HighRes CM-600 column 5 x 1 ml 471204 £126.00
  1 x 5 ml 471205 £91.00
  1 x 10 ml 471206 £134.00
  1 x 20 ml 471207 £215.00


>>Click here for SepFast HighRes Q Products

>>Click here for SepFast HighRes DEAE Products

>>Click here for SepFast HighRes S Products

>>Click here for SepFast HighRes CM Products

How to order

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Data Sheet

Data sheet