IEX SepFastTM HighRes Media

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Q (DEAE, S, CM) SepFast™ HighRes is a group of ion-exchange chromatography media with reduced bead size (20 – 50 μm). They are specially designed for high resolution purification of biological molecules in which impure components are hard to separate by normal bioprocessing chromatography media.

 The core advantages are:

  • High resolution at high loading
  • High sample loading capacity at high flow
  • High productivity

The base matrix of IEX SepFast HighRes is made of highly cross-linked agarose. The media is very stable to most of the chemical conditions experienced in the bioprocessing industry.

Characteristics of IEX SepFast HighRes

SepFast HighRes
SepFast HighRes
SepFast HighRes
SepFast HighRes
Quaternary ammonium strong anion Diethyl-aminoethyl weak anion Sulfo strong cation Carboxy-
methyl weak cation
Total ionic capacity 0.09-0.15 mmol/ml 0.08-0.13 mmol/ml 0.09-0.15 mmol/ml 0.08-0.13 mmol/ml
Particle size 20 – 50 μm
Binding capacity* >100 mg/ml BSA >100 mg/ml BSA >20 mg/ml hIgG >100 mg/ml lysozyme
Pressure-flow property* To be run at a residence time of 2 min and above.
Operational pressure Up to 3 bar
pH stability 2-14 (short term) and 3-12 (long term)
Working temperature 4oC – 30oC
All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol
Total ionic capacity Oxidizing agents,
anionic detergents
Oxidizing agents,
cationic detergents
Storage 20% ethanol 20% ethanol 20% ethanol
+ 0.2M sodium acetate
20% ethanol

*Measured in a 32 mm ID column at a bed height of 15 cm.

Ordering information

Ordering information
Description Quantity Code no. Price GBP
Q SepFast HighRes 25 ml 470201-25ML 103.95
  100 ml 470201-100ML 278.25
  1 Litre 470201-1L POR
  5 Litre 470201-5L POR
  50 Litre 470201-50L POR
DEAE SepFast HighRes 25 ml 470501-25ML 103.95
  100 ml 470501-100ML 278.25
  1 Litre 470501-1L POR
  5 Litre 470501-5L POR
  50 Litre 470501-50L POR
S SepFast HighRes 25 ml 470801-25ML 103.95
  100 ml 470801-100ML 278.25
  1 Litre 470801-1L POR
  5 Litre 470801-5L POR
  50 Litre 470801-50L POR
CM SepFast HighRes 25 ml 471101-25ML 103.95
  100 ml 471101-100ML 278.25
  1 Litre 471101-1L POR
  5 Litre 471101-5L POR
  50 Litre 471101-50L POR

*Other volume sizes are available on request.

How to order