IEX SepFastTM Large Beads Media

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Q , DEAE , SP and CM SepFast Large Beads are a group of ion exchange chromatography media with large particle sizes (100 – 350 μm). The base matrix shows excellent flow property and stable physical property.

The above ion-exchange chromatography media is particularly suitable for large-scale purification of biological molecules from viscous and/or crude culture broths or for high speed flow-through polishing applications in which impure components are chromatographically adsorbed from the main product stream.

This group of ion-exchange media has a balanced design among ligand density, loading capacity and separation power of individual components for large-scale biomanufacturing applications.

The base matrix of SepFast Large Beads is made of highly cross-linked agarose with excellent flow property. They are very stable to most of the chemical conditions experienced in the bioprocessing industry.

Characteristics of IEX SepFast Large Beads

SepFast Large Beads
SepFast Large Beads
SepFast Large Beads
SepFast Large Beads
Matrix Highly cross-linked agarose beads
Quaternary ammonium strong anion Diethyl-aminoethyl weak anion Sulfo strong cation Carboxy-
methyl weak cation
Total ionic capacity 0.09-0.15 mmol/ml 0.08-0.13 mmol/ml 0.09-0.15 mmol/ml 0.08-0.13 mmol/ml
Particle size 100 – 350 μm
Pressure-flow property* >2000 cm/h 
Operational pressure Up to 3 bar
pH stability 2-14 (short term) and 3-12 (long term)
Working temperature 4oC – 30oC
All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol
Total ionic capacity Oxidizing agents,
anionic detergents
Oxidizing agents,
cationic detergents
Storage 20% ethanol 20% ethanol 20% ethanol
+ 0.2M sodium acetate
20% ethanol

*Measured in a 32 mm ID column at a bed height of 15 cm.

Ordering information

Ordering information
Description Quantity Code no. Price GBP
Q SepFast Large Beads 25 ml 440101-25ML 50.40
  100 ml 440101-100ML 147.00
  1 Litre 440101-1L POR
  5 Litre 440101-5L POR
  50 Litre 440101-50L POR
DEAE SepFast Large Beads 25 ml 440301-25ML 50.40
  100 ml 440301-100ML 147.00
  1 Litre 440301-1L POR
  5 Litre 440301-5L POR
  50 Litre 440301-50L POR
SP SepFast Large Beads 25 ml 440501-25ML 50.40
  100 ml 440501-100ML 147.00
  1 Litre 440501-1L POR
  5 Litre 440501-5L POR
  50 Litre 440501-50L POR
CM SepFast Large Beads 25 ml 440701-25ML 50.40
  100 ml 440701-100ML 147.00
  1 Litre 440701-1L POR
  5 Litre 440701-5L POR
  50 Litre 440701-50L POR

*Other volume sizes are available on request.

How to order