IEX SepFastTM Large Beads Media
>>Introduction | >>Product Charactistics | >>Ordering Info | >>How to Order |
---|
Introduction
Q , DEAE , SP and CM SepFast Large Beads are a group of ion exchange chromatography media with large particle sizes (100 – 350 μm). The base matrix shows excellent flow property and stable physical property.
The above ion-exchange chromatography media is particularly suitable for large-scale purification of biological molecules from viscous and/or crude culture broths or for high speed flow-through polishing applications in which impure components are chromatographically adsorbed from the main product stream.
This group of ion-exchange media has a balanced design among ligand density, loading capacity and separation power of individual components for large-scale biomanufacturing applications.
The base matrix of SepFast Large Beads is made of highly cross-linked agarose with excellent flow property. They are very stable to most of the chemical conditions experienced in the bioprocessing industry.
Characteristics of IEX SepFast Large Beads
Q SepFast Large Beads | DEAE SepFast Large Beads | SP SepFast Large Beads | CM SepFast Large Beads | |
Matrix | Highly cross-linked agarose beads | |||
Functionalgroup | Quaternary ammonium strong anion | Diethyl-aminoethyl weak anion | Sulfo strong cation | Carboxy- methyl weak cation |
Total ionic capacity | 0.09-0.15 mmol/ml | 0.08-0.13 mmol/ml | 0.09-0.15 mmol/ml | 0.08-0.13 mmol/ml |
Particle size | 100 – 350 μm | |||
Pressure-flow property* | >2000 cm/h | |||
Operational pressure | Up to 3 bar | |||
pH stability | 2-14 (short term) and 3-12 (long term) | |||
Working temperature | 4oC – 30oC | |||
Chemical stability | All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol | |||
Total ionic capacity | Oxidizing agents, anionic detergents | Oxidizing agents, cationic detergents | ||
Storage | 20% ethanol | 20% ethanol | 20% ethanol + 0.2M sodium acetate | 20% ethanol |
*Measured in a 32 mm ID column at a bed height of 15 cm.
Ordering information
Description | Quantity | Code no. | Price GBP |
Q SepFast Large Beads | 25 ml | 440101-25ML | £50.40 |
100 ml | 440101-100ML | £147.00 | |
1 Litre | 440101-1L | POR | |
5 Litre | 440101-5L | POR | |
50 Litre | 440101-50L | POR | |
DEAE SepFast Large Beads | 25 ml | 440301-25ML | £50.40 |
100 ml | 440301-100ML | £147.00 | |
1 Litre | 440301-1L | POR | |
5 Litre | 440301-5L | POR | |
50 Litre | 440301-50L | POR | |
SP SepFast Large Beads | 25 ml | 440501-25ML | £50.40 |
100 ml | 440501-100ML | £147.00 | |
1 Litre | 440501-1L | POR | |
5 Litre | 440501-5L | POR | |
50 Litre | 440501-50L | POR | |
CM SepFast Large Beads | 25 ml | 440701-25ML | £50.40 |
100 ml | 440701-100ML | £147.00 | |
1 Litre | 440701-1L | POR | |
5 Litre | 440701-5L | POR | |
50 Litre | 440701-50L | POR |
*Other volume sizes are available on request.