Antibody Purification
BioToolomics offers a wide range of chromatography media well suited for R&D scale or bioprocessing scale purification of antibodies, particularly monoclonal antibodies.
The choices include alkaline stable affinity media for capture step and unique IEX / mixed-mode ones for intermediate and polishing steps.
Protein A affinity is the current standard for large scale and preparative antibody production. Biotoolomics offers standard protein A and alkaline-stable protein A immobilized to highly cross-linked agarose base matrix giving high binding capacity and excellent flow properties.
Protein A SepFast; Protein A SepFast Large Beads; Protein A SepFast HighRes; Protein A SuperSpin – Standard affinity media for purifying
antibodies.
Protein A SepFast Endure; Protein A SepFast Large Beads Endure; Protein A SepFast HighRes Endure – Affinity media for purifying antibodies. Alkaline-stable (0.5M NaOH), good for cleaning and sanitisation at process scale.
Protein G SepFast; Protein G SepFast Large Beads; Protein G SepFast HighRes – Having affinity to wider sources / classes of antibodies than Protein A.
Protein A SepFast MAG; Protein G SepFast MAG – Magnetic media. Suitable for micro-scale and high-throughput use.
Cation exchange media is widely used in the purification of Antibodies, either as an initial capture and purification step or following Protein A affinity for further purification. Antibodies typically bind to cation exchange media at neutral pH and are selective eluted using a salt gradient.
SP SepFast 6HF Plus – High dynamic binding capacity good for capture step.
SP SepFast HighRes Plus – High Mab loading capacity; high resolution.
SepFast DUO 150 S – Selectively remove impurities smaller than Mab, shelled bead technology based.
BioToolomics develops mixed mode chromatography products tailored to remove host cell protein impurities from monoclonal antibodies in flow-through operational mode.
MabPolish™ Type I, II, III – Type I for general HCP removal; Type II for high molecular weight species removal; Type III for HCP removal in bind-elute mode.
MabPolish™ DUO 150C – Selectively remove impurities smaller than Mab, shelled bead technology based, cation-exchange mixed-mode ligand.