Description
MabPolish DUO is a class of novel resins with inert shell in the outer-layer of the bead (see the diagram below). The shell has a size-exclusion effect that blocks molecules based on their molecular weights. Impurities smaller than a mAb can penetrate the shell and then be captured by the mixed-mode ligand inside the bead. It is a very gentle method with little loss of product.
The figure above shows the effectiveness of blocking IgG by the size-exclusion shell.
Key Features of MabPolishTM DUO
- Shelled anion-exchange mixed-mode resin (150A); Shelled cation-exchange mixed-mode resin (150C)
- The shell can block molecules of 150 KDa, e.g. monoclonal antibodies.
Application Guide
- Flow-through mode to capture impurities smaller than IgGs at a wide range of pH and conductivity.
MabPolish DUO can be used in flow-through mode to remove impurities of less than 150 KDa from monoclonal antibodies or other antibody materials. Examples of the possible application scenario could be:
- After an antibody is first purified by affinity chromatography media (e.g. Protein A, Protein G or Protein L etc);
- After an antibody is first purified by ion-exchangers;
- After an antibody is first processed through precipitation;
- Direct removal of impurities from crude antibody materials
- After an antibody is modified (e.g. conjugated etc)
MabPolish Selection Kit contains 1 ml each of MabPolish Type I, Type II, Type III, MabPolish DUO 150A, 150C.
Pre-packed columns for screening and process development use are available at following sizes: 1 ml, 5 ml, 3.8 ml (7x100mm ID x BH), 9.5 ml (11x100mm ID x BH), 20 ml (16x100mm ID x BH), 53 ml (26x100mm ID x BH). All these columns have connections fully compatible to Akta systems. Column hardware has 6 bar (0.6 MPa) pressure rating. See Column Instruction for details of HiSep and Optio column hardware.
Pre-packed columns for pilot to production scale use with column diameter ranging from 5cm to 45cm are available on request. For bulk loose media, please contact us.
This range of chromatography media is developed and supported for production scale chromatography use. Regulatory Support File (RSF) is available to assist process validation and submissions to regulatory authorities.