Description
Protein G SepFast Media is a family of affinity resins with protein G immobilised to highly cross-linked agarose-based beads of various sizes.
- Protein G SepFast HighRes: 20 – 50 µm, good for small column run at shorter residence time (i.e. higher flowrate)
- Protein G SepFast: 50 – 150 µm, good for most applications from small scale to large scale purification requirements (i.e. low column pressure);
- Protein G SepFast Large Bead: 150 – 350 µm, particularly good for processing viscous or cell-containing feedstocks (i.e. high settling rate and extremely low column pressure)
Protein G binds to the Fc region of IgG from a variety of mammalian species. Protein G SepFast may be used to isolate and purify classes, subclasses and fragments of Immunoglobulins from any biological fluid or cell culture medium. Protein G SepFast is extremely useful for isolation of immune complexes.
The potential applications of protein G include practically all of the current and projected applications of protein A. Protein G and protein A, however, have different IgG binding specificities, dependent on the origin of the IgG. Compared to protein A, protein G binds more strongly to polyclonal IgG, for example, from cow, sheep and horse. Furthermore, unlike protein A, protein G binds polyclonal rat IgG, human IgG3 and mouse IgG1.
Protein G SepFast is developed and supported for production scale chromatography. BioToolomics offers both loose medium and pre-packed ready-to-use disposable columns.