Packing Disposable Chromatography Columns

For predictable screening and scaling-up chromatography purification steps, reliable and reproducible column packing is very critical. We have extensive experiences in handling a broad range of process chromatography media, from soft to rigid, from small particles to large particles, from light particles to dense particles and from polymer-based particles to inorganic particles. Resins can be of any type you choose.

We can pack high performance and robust disposable chromatography columns from small to large (e.g. 1μl to >30 litres), for cGMP or non-cGMP applications, using our proprietary column design. The bed height can be anywhere up to 60 cm.

Our range of chromatography columns has the following key features and advantages:

• Fully scalable column design for bioprocessing applications
• Low cost
• Resin compressibility taken into account
• Minimum dead volume for high resolution applications
• Proven flow distribution mechanism
• Disposable
• Tight packing of any type of chromatography media (>20 µm)
• Features standard connections, which are compatible with most common chromatography instruments such as AKTA etc.
• Easy to store
• Long life time

For each packed column we will test HETP, Peak Asymmetry and Flow Properties (if required). For cGMP grade columns, in addition to the above tests, we will sanitise the columns and also test endotoxin and bioburden levels.

We can pack a column according to your protocol or develop the best predictable packing process for you. According to your special applications, we can also tailor our column design to suit your needs

The available column sizes and their characteristics can be seen in the tables below.

Please contact us to discuss your requirements in details.

We offer the following packing services for medium to high throughput purification applications:

Product Type	Packed Volume	Materials of Construction	Operational Pressure	Chemical Compatibility
96 well filter plate	1 μl to 500 μl	Polypropylene & PVDF	N/A	All products are compatible with most commonly used aqueous buffers. For special chemical or certain organic solvent compatibility please contact us.
Spin column	20 μl to 500 μl	Polypropylene	N/A
1 ml column	1 ml	Polypropylene	5 bar
5 ml column	5 ml	Polypropylene & acrylic	5 bar

We offer the following packing services for viral clearance study, process development and manufacture applications:

Inner diameter (mm)	Typical volume	Connection	Pressure rating	Application
5	0.2 ml – 4 ml	1/16” tubing connection, compatible to all the common chromatography systems such as AKTA etc;   Or mini tri-clamp connection (for 11mm – 50mm diameter GMP grade columns)	6 bar (84 psi)	Preparative purifications Process development & validation Viral clearance study non-GMP production GMP production (available with 11mm to 450mm diameter columns); the wet-contact parts of GMP grade columns meet USP and FDA regulatory standards with traceability
6	0.2 ml – 1 ml
7	0.4 ml – 8 ml
11	1 ml – 20 ml
16	2 ml – 60 ml
26	5 ml – 100 ml
50	100 ml – 400 ml
80	0.25 litre – 1 litre	Mini tri-clamp	5 bar (70 psi)
100	0.5 litre – 1.6 litre
129	1 litre – 2.5 litre
200	1.5 litre – 6 litre
240	2 litre – 10 litre
330	5 litre – 20 litre
450	15 litre – 32 litre	Mini tri-clamp	3 bar (42 psi)