Description
Phenyl SepFast™ range of hydrophobic interaction chromatography (HIC) media with carbon ring of C6 has mild hydrophobicity and possible other interactions such as π- π or polar interactions.
The feature and selection guide is listed as follows:
| 4HF serial (50µm – 150µm) |
6HF serial (50µm – 150µm) |
Large Beads (150µm – 350µm) |
HighRes (25µm – 50µm) |
| The above HIC media is designed to purify most medium to large proteins | The above HIC media is designed to purify peptides or smaller proteins | The above HIC media is designed to purify proteins from crude or viscous samples | The above HIC media is designed to purify proteins that require high resolution |
Pre-packed columns for screening and process development use are available at following sizes: 1 ml, 5 ml, 3.8 ml (7x100mm ID x BH), 9.5 ml (11x100mm ID x BH), 20 ml (16x100mm ID x BH), 53 ml (26x100mm ID x BH). All these columns have connections fully compatible to Akta systems. Column hardware has 6 bar (0.6 MPa) pressure rating. See Column Instruction for details of HiSep and Optio column hardware.
Pre-packed columns for pilot to production scale use with column diameter ranging from 5cm to 45cm are available on request. For bulk loose media, please contact us.
The range of HIC media is developed and supported for production scale chromatography use. Regulatory Support File (RSF) is available to assist process validation and submissions to regulatory authorities.
More information about Phenyl media.
More information about HIC Selection Kit.
HIC Selection Kit contains 7 x 1 ml column (Butyl SepFast HighRes, Butyl SepFast 4HF, Phenyl SepFast HighRes, Phenyl SepFast 6HF, Phenyl SepFast 6HF high sub, Octyl SepFast 4HF, Butyl-S SepFast 4HF, respectively).