Affinity Media

Magnetic IMAC Resins

£130.31£164.08 ex. VAT

Description

Magnetic IMAC (immobilised metal affinity chromatography) resin is typically useful in purifying small quantity low concentration histidine-tagged proteins. For example, when 10 – 50 µl of resin is required for protein purification, it is difficult to handle such small quantities in columns.

Magnetic IMAC resins can be easily processed with the aid of a magnet. The liquid / solid separation required in each purification step (e.g. equilibration, binding, washing and elution) is readily achieved with the aid of a magnet (e.g. magnetic stirring bar or other commercial magnetic devices).

Key benefits:

  • Tailored base matrix design for batch magnetic purification of proteins
  • High binding capacity (could be over 40 mg/ml)
  • Simple and convenient operation, no chromatography training required
  • Flexible operational conditions
  • Low cost
  • A choice of three types of immobilised metal ions available
  • Very low metal leakage
  • Full operation manual (including purification conditions) supplied

Larger pack sizes available on request.

Note: No magnet is supplied with the above products. Customers need to source the magnet themselves.

More information about IMAC SepFast MAG.

More information about Ready-to-Use His Buffer Kit. 

Characteristics of Magnetic IMAC Resins

Particle Size

50 – 150 µm

Matrix

Cross-linked 6% agarose encapsulating magnetic particles

Metal Ion Capacity

At least 15 μmol / ml resin*

*Tested with nickel ion charged

Protein Binding Capacity

Depends on the type of proteins and binding conditions; could be over 40 mg / ml resin*

*Tested with nickel ion charged

Chemical Stability**

Stable in
0.01M HCl and 1% SDS tested for 30 mins;
0.5 M NaOH tested for overnight

**Tested with the absence of metal ion

pH Stability**

2-14 (<2 h)
4-12 (up to one week)

Storage

20% ethanol at 4°C