Ion Exchange (IEX) media

IEX SepFast Large Beads Plus

£116.01£1,218.80 ex. VAT

Description

Q, DEAE , SP and CM SepFast Large Beads Plus are a group of ion exchange chromatography media with large particle sizes (100 – 350 μm) and high binding capacity. The base matrix shows excellent flow property and stable physical property.

The above ion-exchange chromatography media is particularly suitable for large-scale purification of biological molecules from viscous and/or crude culture broths or for high speed flow-through polishing applications in which impure components are chromatographically adsorbed from the main product stream.

This group of ion-exchange media has a balanced design among ligand density, loading capacity and separation power of individual components for large-scale biomanufacturing applications. IEX SepFast Large Beads Plus offers increased binding capacity and faster binding kinetics than IEX SepFast Large Beads.

The SepFast Large Beads Plus is made of highly cross-linked agarose grafted with dextran for increased binding capacity. They are very stable to most of the chemical conditions experienced in the bioprocessing industry.

Further information about IEX SepFast Large Beads Plus.

Characteristics of IEX SepFast Large Beads Plus

Matrix

Highly cross-linked agarose and dextran beads

Functional Group

Quaternary ammonium strong anion (Q SepFast Large Beads Plus)

Diethylaminoethyl weak anion (DEAE SepFast Large Beads Plus)

Sulfopropyl strong cation (SP SepFast Large Beads Plus)

Carboxymethyl weak cation (CM SepFast Large Beads Plus)

Total Ionic Capacity

0.1-0.2 mmol/ml (Q SepFast Large Beads Plus)

0.1-0.2 mmol/ml (DEAE SepFast Large Beads Plus)

0.1-0.2 mmol/ml (SP SepFast Large Beads Plus)

0.1-0.2 mmol/ml (CM SepFast Large Beads Plus)

Particle Size

100 – 350 μm

Operational-Flow Velocity*

>2000 cm/hr

*Measured in a 32 mm ID column at a bed height of 15 cm using water at room temperature.

pH Stability

2-14 (short term) and 3-12 (long term) (Q SepFast Large Beads Plus / DEAE SepFast Large Beads Plus)

2-14 (short term) and 4-12 (long term) (SP SepFast Large Beads Plus / CM SepFast Large Beads Plus)

Working Temperature

+4°C – +30°C

Chemical Stability

All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol

Avoid

Oxidizing agents, anionic detergents (Q SepFast Large Beads Plus / DEAE SepFast Large Beads Plus)

Oxidizing agents, cationic detergents (SP SepFast Large Beads Plus / CM SepFast Large Beads Plus)

Storage

20% ethanol (Q SepFast Large Beads Plus)

20% ethanol (DEAE SepFast Large Beads Plus)

20% ethanol + 0.2M sodium acetate (SP SepFast Large Beads Plus)

20% ethanol (CM SepFast Large Beads Plus)