Description
SepFast SEC 1-10KDa, 3-70KDa, 6-600KDa and 6-5000KDa gel filtration media is specially designed for high resolution and high recovery fractionation of biological molecules based on their molecular weights. Gel filtration (or called size exclusion chromatography, SEC) is a proven technique, which is widely used for size-based molecular separations.
SepFast gel filtration media has a balanced design to offer high recovery and high selectivity according to individual applications. The core advantages are:
- High resolution
- High selectivity
- High recovery
- Highly scalable
- Excellent physical and mechanical stability
- Cost effective
The base matrix is made of agarose or a composite of polysaccharides that have been highly cross-linked. The media is very stable to most of the chemical conditions experienced in the bioprocessing industry.
Pre-packed columns are available at following sizes: 11x300mm ID x BH, 16x600mm ID x BH, 26x100mm ID x BH. All these columns have connections fully compatible to Akta systems. Column hardware has 6 bar (0.6 MPa) pressure rating. Larger diameter columns at up to 60-100cm bed height are available on request.
Examples of references featuring SepFast gel filtration (SEC) Products:
de Cassan, S.C., Shakri, A.R., Llewellyn, D., Elias, S.C., Cho, J.S., Goodman, A.L., Jin, J., Douglas, A.D., Suwanarusk, R., Nosten, F.H. and Rénia, L., 2015. Preclinical assessment of viral vectored and protein vaccines targeting the Duffy-binding protein region II of Plasmodium vivax. Frontiers in immunology, 6, p.348
Jin, J., Tarrant, R.D., Bolam, E.J., Angell-Manning, P., Soegaard, M., Pattinson, D.J., Dulal, P., Silk, S.E., Marshall, J.M., Dabbs, R.A. and Nugent, F.L., 2018. Production, quality control, stability, and potency of cGMP-produced Plasmodium falciparum RH5. 1 protein vaccine expressed in Drosophila S2 cells. NPJ vaccines, 3(1), p.32
More information about SepFast SEC 1-10KDa.
More information about SepFast SEC 3-70KDa and 6-600KDa.
More information about SepFast SEC 6-5000KDa.
More information about pre-packed SepFast SEC columns (11x300mm, 16x600mm, 26x600mm).