Hydrophobic Interaction Chromatography (HIC) Media

Butyl Media

£132.66£1,467.43 ex. VAT

Description

Butyl SepFast™ range of hydrophobic interaction chromatography (HIC) media with carbon chain of C4 has quite mild hydrophobicity. 

Butyl-S media uses thiol ether linker to the base matrix instead of standard ether linker, which may change the polarity of the ligand to offer certain unique selectivity. 

The feature and selection guide is listed as follows:

4HF serial
(50µm – 150µm)
6HF serial
(50µm – 150µm)
Large Beads
(150µm – 350µm)
HighRes
(25µm – 50µm)
The above HIC media is designed to purify most medium to large proteins The above HIC media is designed to purify peptides or smaller proteins The above HIC media is designed to purify proteins from crude or viscous samples The above HIC media is designed to purify proteins that require high resolution

Pre-packed columns for screening and process development use are available at following sizes: 1 ml, 5 ml, 3.8 ml (7x100mm ID x BH), 9.5 ml (11x100mm ID x BH), 20 ml (16x100mm ID x BH), 53 ml (26x100mm ID x BH). All these columns have connections fully compatible to Akta systems. Column hardware has 6 bar (0.6 MPa) pressure rating. See Column Instruction for details of HiSep and Optio column hardware. 

Pre-packed columns for pilot to production scale use with column diameter ranging from 5cm to 45cm are available on request. For bulk loose media, please contact us.

The range of HIC media is developed and supported for production scale chromatography use. Regulatory Support File (RSF) is available to assist process validation and submissions to regulatory authorities.

More information about Butyl media.

More information about HIC Selection Kit.

HIC Selection Kit contains 7 x 1 ml column (Butyl SepFast HighRes, Butyl SepFast 4HF, Phenyl SepFast HighRes, Phenyl SepFast 6HF, Phenyl SepFast 6HF high sub, Octyl SepFast 4HF, Butyl-S SepFast 4HF, respectively).

Characteristics of Butyl Media

Matrix

Highly cross-linked agarose; 4% agarose for SepFast 4HF, 6% agarose for SepFast 6HF

Functional Group

-(CH₂)₃CH₃

Ligand Density

Around 40 µmol/ml

Binding Capacity

>20 mg lyzosyme / ml

Particle Size

25 – 50 µm (HighRes), 50 – 150 µm (4HF and 6HF), 100 – 350 µm (Large Beads)

Pressure-Flow Property*

>100 cm/h for HighRes;
>300 cm/h for 4HF serials;
>500 cm/h for 6HF serials;
>1500 cm/h for Large Beads

*Measured in a 32 mm ID column at a bed height of 15 cm.

Operational Pressure

Up to 3 bar

pH Stability

2-14 (short term) and 3-12 (long term)

Working Temperature

4°C – 30°C

Chemical Stability

All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% acetonitrile, 30% isopropanol, 70% ethanol, 2 M (NH4)2SO4

Storage

20% ethanol