IMAC SuperSpin tubes

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Introduction

With a patented novel technology (patent pending), IMAC SuperSpin tube is supplied as a low cost, single use disposable device that allows rapid purification and screening of histidine-tagged proteins at much less cost. The spin tube is filled with novel metal immobilised chromatography resin of small particles (20 – 50 µm). It gives fast mass transfer rate of target proteins. So decent amount of protein can be captured in a short solid / liquid contact time. Four types of spin tubes are available as Ni SuperSpin, Cu SuperSpin, Co SuperSpin and Zn SuperSpin for fast screening of the best immobilised metal ion to a given target protein. It is a particularly powerful tool in applications such as small-scale purification, high-throughput screening, purification process optimisation etc.

Key benefits:

  • Fast purification of tens of protein samples with a standard microcentrifuge
  • High protein binding capacity, as very small IMAC particle with slow settling rate is employed to give fast mass transfer
  • Very low cost in comparison to similar product in the marketplace
  • Optimisation of the binding, washing and elution conditions (e.g. imidazole concentration, pH, salt concentration, detergents etc) in one or two sets of experiments at short period and very low cost
  • Direct processing of clarified or unclarified protein sample
  • Full operational manual supplied

The fine IMAC particles are very stable and compatible to a variety of chemical reagents (e.g. denaturing reagents and reducing reagents etc).

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Characteristics of Ni SuperSpin / Cu SuperSpin / Co SuperSpin / Zn SuperSpin

Product characteristics
Tube material Polypropylene
Medium IMAC SepFast
Particle size 20 – 50 µm
Packed volume 50 µl
Protein binding capacity Depends on the type of proteins and binding conditions;
up to 800 µg*
Chemical compatibility Stable in the commonly used buffers and denaturing reagents, avoid chelating reagents e.g. EDTA, EGTA and citrate
Storage condition 2oC – 8oC

*Tested with nickel ion charged

Ordering information

Ordering information
Product Quantity Code no. Price GBP
Ni SuperSpin 50 150101 £92.00
Cu SuperSpin 50 150102 £92.00
Co SuperSpin 50 150103 £92.00
Zn SuperSpin 50 150104 £92.00
Ordering information
Related products Quantity Code no. Price GBP
SuperSpin Desaltor 50 210101 £115.00
Ready-to-use His Buffer Kit 2*50ml stock phosphate buffer and 50ml stock imidazole solution 200105 £39.00

How to order

Products

Ni SuperSpin: Purification of a 6 x His tagged hydrolase from clarified E.coli. cell lysate with a microcentrifuge

Lane 1: Molecular marker;
Lane 2: clarified cell lysate;
Lane 3: Cell lysate pass through;
Lane 4: Wash with binding buffer (20 mM phosphate, 20 mM imidazole and 500 mM NaCl, pH 7.4);
Lane 5: Second wash with the same buffer;
Lane 6 and 7: Eluates at 500 mM imidazole in the binding buffer, pH 7.4.

Ni Superspin

Operation Manual

Operation Manual

MSDS