IMAC SepFast BG
| >>Introduction | >>Product Charactistics | >>Ordering Info | >>How to Order |
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Introduction
IMAC SepFast BG is unique as it is specifically designed for the batch and/or gravity flow purifications of histidine-tagged proteins. It can be used in packed bed operation or applied to 96-well microplates as well. It is produced through a novel process that gives great reduction in manufacturing cost. As a result, our customers can enjoy a great decrease in their purification cost.
Key benefits:
- Best performance : price ratio for batch or gravity flow purification
- Low product cost due to the novel manufacturing processes
- High binding capacity (could be over 40 mg/ml)
- Fast and easy protein purification from unclarified or clarified samples
- All purification steps (binding, washing and elution etc) be done in a single column (supplied) no matter it is batch or gravity flow operation
- No expensive chromatography system or column is required
- Choice of four pre-charged metal ions
- Very low metal leakage
- Full protein purification manual supplied
Its rough surface and carefully controlled pore structure allow fast access of the immobilised metal ligand by target protein molecules. Also, its high mechanical strength permits liquid passing through a column at decent flow rates. Clarified or unclarified cell lysates (for intracellular proteins) or culture broths (for extracellular proteins) can be directly processed with IMAC SepFast BG. All these translate into high process flexibility and higher protein yield at shortened purification time.
To facilitate the purification process with as little hassle as possible in batch or gravity flow operations, two specially designed empty columns can be ordered in separate. The larger column BG-30 has a working volume of 30 ml with volumetric marks in the first 20 ml (at 1 ml interval). The smaller one BG-5 has a working volume of up to 5 ml. There is a sintered porous mesh underneath each column to withhold chromatography particles. Both lids in the bottom and the top of the column are provided. Therefore, the whole protein purification process, i.e. resin equilibration, binding, washing, elution and regeneration (or clean-in-place) can be done within the same column in a very economic way. For further process flexibility, the desired volume of resin can be loaded to the column of choice without any restriction. The smaller column is ideal for working resin volume of 0.1ml to 1 ml. The larger one is ideal for resin volume of 1 ml to 10 ml.
IMAC SepFast BG or its four metal charged formats i.e. Ni SepFast BG, Cu SepFast BG, Co SepFast BG and Zn SepFast BG, is highly stable and compatible to a wide range of chemicals (e.g. detergents, denaturing reagents and reducing reagents etc) commonly experienced in protein purification processes, which means that more flexible operations can be developed for the best performance.
A screening kit containing four types of immobilised metal ions is available for scouting the best metal ion to given proteins.
Product Characteristics
| Particle size | 50 – 150 µm |
| Base matrix | Cross-linked 6% agarose |
| Metal ion capacity | Approx. 15 - 20 µmol / ml resin |
| Protein binding capacity | Depends on the type of proteins and binding conditions; could be over 40 mg/ml resin* |
| Chemical stability** | Stable in0.1M HCl and 1% SDS tested for 30 mins;0.5 M NaOH and 30% acetic acid tested for overnight; 0.01M HCl, 0.1M NaOH and 0.2M acetic acid tested for one week. |
| pH stability** | 2-14 (<2 h)3-12 (up to one week) |
| Storage | 20% ethanol at 4oC |
*Test with nickel ion charged; **Tested with the absence of metal ions.
Ordering information
| Product | Quantity | Code no. | Price GBP |
| IMAC SepFast BG | 10 ml | 180112 | £81.00 |
| 25 ml | 180101 | £133.00 | |
| 100 ml | 180102 | £473.00 | |
| Screening kit of IMAC SepFast BG | 5 ml each of Ni SepFast BG, Cu SepFast BG, Co
SepFast BG and Zn SepFast BG, respectively; plus 1 Ready-to-use His Buffer Kit |
180103 | £198.00 |
| Ni SepFast BG | 10 ml | 180113 | £84.00 |
| 25 ml | 180104 | £149.00 | |
| 100 ml | 180105 | £552.00 | |
| Cu SepFast BG | 25 ml | 180106 | £149.00 |
| 100 ml | 180107 | £552.00 | |
| Co SepFast BG | 25 ml | 180108 | £149.00 |
| 100 ml | 180109 | £552.00 | |
| Zn SepFast BG | 25 ml | 180110 | £149.00 |
| 100 ml | 180111 | £552.00 |
| Columns | Quantity | Code no. | Price GBP |
| BG-30 column | 20 | 200101 | £77.00 |
| BG-30 column | 50 | 200102 | £151.00 |
| BG-5 column | 20 | 200103 | £71.00 |
| BG-5 column | 50 | 200104 | £136.00 |
| Related products | Quantity | Code no. | Price GBP |
| Ready-to-use His Buffer Kit | 2*50ml stock phosphate buffer and 50ml stock imidazole solution | 200105 | £43.00 |
| SuperSpin Desaltor | 50 | 210101 | £115.00 |
How to order
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Ni SepFast BG: Purification of a 6 x His tagged hydrolase from unclarified E.coli. cell lysate in a batch operation
Lane 1: Molecular marker; Lane 2: Unclarified cell lysate; Lane 3: Cell lysate pass through; Lane 4: Wash with binding buffer (20 mM phosphate, 20 mM imidazole and 500 mM NaCl, pH 7.4); Lane 5: Wash with 50 mM imidazole in the binding buffer; Lane 6 and 7: Eluates at 500 mM imidazole in the binding buffer, pH 7.4.
Ni SepFast BG
