Protein A SuperSpin
Introduction
Protein A SuperSpin is supplied for fast small-scale purification of
immunoglobulin G (IgG) from various species (e.g. serum, ascites and mammalian
cell culture broth etc). Protein A has been well proven a powerful affinity
ligand which shows very high specificity to IgGs from mammalian hosts.
Each Protein A SuperSpin tube accommodates 50 µl of Protein A agarose particles.
The affinity resin is specifically designed to provide large pore size with
small particle size (i.e. 20 – 50 µm). So large antibody molecules can access
the affinity ligand rapidly with excellent mass transfer rate. As a result, half milligram quantity of antibody can be easily purified within
20 minutes with
inexpensive cost. No special expensive chromatography instrument and column is
required.
Protein A SuperSpin is particularly useful for the following applications:
- The quantity of antibody to be purified is small (less than half milligram)
- On-line detection of antibody expression level
- High throughput purification of antibodies
- High throughput screening of antibodies
Key benefits:
- Rapid antibody capture and recovery
- High antibody binding capacity (half milligram)
- High throughput format (24 samples can be processed in parallel)
- Low cost
- Easy to use and no expensive equipment required
>>>For more info...
Characteristics of Ni SuperSpin / Cu SuperSpin / Co SuperSpin / Zn SuperSpin
Product characteristics
| Tube material |
Polypropylene |
| Resin |
Protein A SepFast |
| Particle size |
20 – 50 µm |
| Packed volume |
50 µl |
| Protein binding capacity |
Depends on the type of proteins and binding conditions; up to 500
µg |
| Chemical compatibility |
The resin maintains the binding capability after exposing to 3M NaSCN,
6M GuHCl or 8M urea over a few hours |
| Storage condition |
2oC – 8oC |
Ordering information
Ordering information
| Product |
Quantity |
Code no. |
Price GBP |
| Protein A SuperSpin |
20 |
230101 |
£96.00 |
| Protein A SuperSpin |
50 |
230102 |
£199.00 |
Ordering information
| Related products |
Quantity |
Code no. |
Price GBP |
| SuperSpin Desaltor |
50 |
210101 |
£85.00 |
| SuperSpin Concentrator |
50 |
220101 |
£112.00 |