Chelating SepFast BG
| >>Introduction | >>Product Charactistics | >>Ordering Info | >>How to Order |
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Introduction
Chelating SepFast BG employs IDA (iminodiacetic acid) as the metal chelating ligand. It is covalently coupled to porous agarose base matrix. Chelating SepFast BG possesses high mechanical strength and high mass transfer rate. When the requirement of target protein purity and/or protein binding capacity isn’t high, Chelating SepFast BG is an ideal choice for low cost purification of His-tagged proteins. It is recommended in batch or gravity flow purification operations. Empty BG-30 and BG-5 column can be used for such applications. The operation is simple and no expensive equipment is required.
Product Characteristics
| Particle size | 50 – 150 µm |
| Base matrix | Highly cross-linked 6% agarose |
| Metal ion capacity | Approx. 20 - 30 µmol / ml resin* |
| Chemical stability** | Stable in0.1M HCl and 1% SDS, 30 mins;0.5 M NaOH and 30% acetic acid, overnight; 0.01M HCl, 0.1M NaOH and 0.2M acetic acid, one week. |
| pH stability** | 2-14 (<2 h)3-12 (within one week) |
| Storage | 20% ethanol at 4oC |
*Test after copper ion is charged; **Tested with the absence of metal ions.
Ordering information
| Columns | Quantity | Code no. | Price GBP |
| Chelating Sepfast BG | 25ml | 240101 | £81.00 |
| BG-30 column | 20 | 200101 | £77.00 |
| BG-30 column | 50 | 200102 | £151.00 |
| BG-5 column | 20 | 200103 | £71.00 |
| BG-5 column | 50 | 200104 | £136.00 |
| Ready-to-use His Buffer Kit | 2*50ml stock phosphate buffer and 50ml stock imidazole solution | 200105 | £43.00 |
How to order
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