IMAC SuperSpin tubes
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IMAC SuperSpin is supplied as a low cost disposable spin-column that allows rapid purification and screening of histidine-tagged proteins at a low cost. The spin tube is filled with novel metal immobilised chromatography resin of small particles (20 – 50 µm). It gives fast mass transfer rate of target proteins, so decent amounts of protein can be captured in a short solid / liquid contact time. Three types of spin tubes are available; Ni SuperSpin, Co SuperSpin and Zn SuperSpin, for fast screening of the best immobilised metal ion to a given target protein. It is a particularly powerful tool in applications such as small-scale purification, high-throughput screening, purification process optimisation etc.
- Fast purification of many protein samples with a standard microcentrifuge
- High protein binding capacity
- Very low cost in comparison to similar products in the marketplace
- Optimisation of the binding, washing and elution conditions (e.g. imidazole concentration, pH, salt concentration, detergents etc) in one or two sets of experiments in short periods and at very low cost
- Direct processing of clarified or unclarified protein sample
- Full operational manual supplied
The fine IMAC particles are very stable and compatible with a variety of chemical reagents (e.g. denaturing reagents and reducing reagents etc).
Characteristics of Ni SuperSpin / Co SuperSpin / Zn SuperSpin
|Particle size||20 – 50 µm|
|Packed volume||50 µl|
|Protein binding capacity||Depends on the type of proteins and binding conditions;
up to 800 µg*
|Chemical compatibility||Stable in the commonly used buffers and denaturing reagents, avoid chelating reagents e.g. EDTA, EGTA and citrate|
|Storage condition||2oC – 8oC|
*Tested with nickel ion charged
|Product||Quantity||Code no.||Price GBP|
|Related products||Quantity||Code no.||Price GBP|
|Ready-to-use His Buffer Kit||2*50ml stock phosphate buffer and 50ml stock imidazole solution||200105||£61.64|
Ni SuperSpin: Purification of a 6 x His tagged hydrolase from clarified E.coli. cell lysate with a microcentrifuge
Lane 1: Molecular marker; Lane 2: clarified cell lysate; Lane 3: Cell lysate pass through; Lane 4: Wash with binding buffer (20 mM phosphate, 20 mM imidazole and 500 mM NaCl, pH 7.4); Lane 5: Second wash with the same buffer; Lane 6 and 7: Eluates at 500 mM imidazole in the binding buffer, pH 7.4.