IMAC SepFast

>>Introduction	>>Product Charactistics	>>Ordering Info	>>How to Order

Introduction

IMAC SepFast is designed for the purification of histidine-tagged proteins in batch, gravity flow, packed bed, or 96-well microplate operations.

Key benefits:

Low cost due to the novel manufacturing processes
High binding capacity (could be over 40 mg/ml)
Fast and easy protein purification from unclarified or clarified samples
Choice of three pre-charged metal ions
Very low metal leakage
Full protein purification manual supplied

Its carefully controlled pore structure allows fast access of the target protein to the immobilised metal ligand. Also, its high mechanical strength permits liquid to pass through a column at excellent flow rates. Clarified or unclarified cell lysates (for intracellular proteins) or culture broths (for extracellular proteins) can be directly processed with IMAC SepFast. All these translate into high process flexibility and higher protein yield at a shortened purification time.

IMAC SepFast or its three metal charged formats i.e. Ni SepFast, Co SepFast and Zn SepFast, are highly stable and compatible with a wide range of chemicals (e.g. detergents, denaturing reagents and reducing reagents etc) commonly used in protein purification processes, which means that more flexible operations can be developed for the best performance.

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Product Characteristics

Product characteristics
Particle size	50 – 150 µm
Base matrix	Cross-linked 6% agarose
Metal ion capacity	Approx. 12 - 25 µmol / ml resin
Protein binding capacity	Depends on the type of proteins and binding conditions; could be over 40 mg/ml resin*
Chemical stability**	Stable in 0.1M HCl and 1% SDS tested for 30 mins; 0.5 M NaOH and 30% acetic acid tested for overnight; 0.01M HCl, 0.1M NaOH and 0.2M acetic acid tested for one week.
pH stability**	2-14 (<2 h) 3-12 (up to one week)
Storage	20% ethanol at 4^oC

*Test with nickel ion charged; **Tested with the absence of metal ions.

Ordering information

Ordering information
Product	Quantity	Code no.	Price GBP
IMAC SepFast	10 ml	180101-10ML	£115.22
	25 ml	180101-25ML	£188.97
	100 ml	180101-100ML	£624.75
Ni SepFast	10 ml	180201-10ML	£119.29
	25 ml	180201-25ML	£211.80
	100 ml	180201-100ML	£783.99
Co SepFast	25 ml	180301-25ML	£211.80
	100 ml	180301-100ML	£783.99
Zn SepFast	25 ml	180401-25ML	£211.80
	100 ml	180401-100ML	£783.99

Ordering information
Related products	Quantity	Code no.	Price GBP
Ready-to-use His Buffer Kit	2*50ml stock phosphate buffer and 50ml stock imidazole solution	200105	£61.64
SuperSpin Desaltor	50	210101	£162.29

How to order

Ni SepFast: Purification of a 6 x His tagged hydrolase from unclarified E.coli. cell lysate in a batch operation

Lane 1: Molecular marker;
Lane 2: Unclarified cell lysate;
Lane 3: Cell lysate pass through;
Lane 4: Wash with binding buffer (20 mM phosphate, 20 mM imidazole and 500 mM NaCl, pH 7.4);
Lane 5: Wash with 50 mM imidazole in the binding buffer;
Lane 6 and 7: Eluates at 500 mM imidazole in the binding buffer, pH 7.4.