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IMAC SepFast is designed for the purification of histidine-tagged proteins in batch, gravity flow, packed bed, or 96-well microplate operations.
- Low cost due to the novel manufacturing processes
- High binding capacity (could be over 40 mg/ml)
- Fast and easy protein purification from unclarified or clarified samples
- Choice of three pre-charged metal ions
- Very low metal leakage
- Full protein purification manual supplied
Its carefully controlled pore structure allows fast access of the target protein to the immobilised metal ligand. Also, its high mechanical strength permits liquid to pass through a column at excellent flow rates. Clarified or unclarified cell lysates (for intracellular proteins) or culture broths (for extracellular proteins) can be directly processed with IMAC SepFast. All these translate into high process flexibility and higher protein yield at a shortened purification time.
IMAC SepFast or its three metal charged formats i.e. Ni SepFast, Co SepFast and Zn SepFast, are highly stable and compatible with a wide range of chemicals (e.g. detergents, denaturing reagents and reducing reagents etc) commonly used in protein purification processes, which means that more flexible operations can be developed for the best performance.
|Particle size||50 – 150 µm|
|Base matrix||Cross-linked 6% agarose|
|Metal ion capacity||Approx. 12 - 25 µmol / ml resin|
|Protein binding capacity||Depends on the type of proteins and binding conditions;
could be over 40 mg/ml resin*
|Chemical stability**||Stable in0.1M HCl and 1% SDS tested for 30 mins;0.5 M NaOH and 30% acetic acid tested for overnight; 0.01M HCl, 0.1M NaOH and 0.2M acetic acid tested for one week.|
|pH stability**||2-14 (<2 h)3-12 (up to one week)|
|Storage||20% ethanol at 4oC|
*Test with nickel ion charged; **Tested with the absence of metal ions.
|Product||Quantity||Code no.||Price GBP|
|IMAC SepFast||10 ml||180101-10ML||£115.22|
|Ni SepFast||10 ml||180201-10ML||£119.29|
|Co SepFast||25 ml||180301-25ML||£211.80|
|Zn SepFast||25 ml||180401-25ML||£211.80|
|Related products||Quantity||Code no.||Price GBP|
|Ready-to-use His Buffer Kit||2*50ml stock phosphate buffer and 50ml stock imidazole solution||200105||£61.64|
Ni SepFast: Purification of a 6 x His tagged hydrolase from unclarified E.coli. cell lysate in a batch operation
Lane 1: Molecular marker; Lane 2: Unclarified cell lysate; Lane 3: Cell lysate pass through; Lane 4: Wash with binding buffer (20 mM phosphate, 20 mM imidazole and 500 mM NaCl, pH 7.4); Lane 5: Wash with 50 mM imidazole in the binding buffer; Lane 6 and 7: Eluates at 500 mM imidazole in the binding buffer, pH 7.4.