Pre-packed Ni SepFastTM
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Introduction
Ni SepFast resin is packed into our robust and durable
chromatography columns for convenient use. The column ends have 1/16" fittings
that are compatible with common chromatography systems (such as AKTA). The
column body is robust enough for use in moderate pressure operations. The column
has been well designed to minimise the dead volume and gives an excellent flow
distribution across the bed.
The 1 ml column is made of polypropylene. The column body for the 5 ml column is
made of acrylic, which gives a clear view of the packed resin.
Key benefits:
- Low cost
- High protein binding capacity with high purity
- Ready to use
- Robust and durable for re-use or longer storage
- Full operational manual supplied
We can also pack other type of chromatography resins that you choose into our columns with a reduced cost. Larger column sizes are also available. See our Column Packing Service for more information.
For large quantities of columns, please contact us directly and we will offer a very good price structure to satisfy your applications.
Characteristics of Pre-packed Ni SepFast Columns
Column material | Polypropylene / acrylic |
Medium | Ni SepFast |
Operating pressure | 4 bar or less |
Packed volume | 1 ml, 5 ml |
Protein binding capacity | Depends on the type of proteins and binding conditions; up to 40 mg/ml* |
Chemical compatibility | Stable in the commonly used buffers and denaturing reagents, avoid chelating reagents e.g. EDTA, EGTA and citrate |
Storage condition | 4oC – 30oC |
*Tested with nickel ion charged
Ordering information
Product | Quantity | Code no. | Price GBP |
Pre-packed Ni SepFast Column | 1 x 1 ml | 180201-1x1ML | £32.41 |
Pre-packed Ni SepFast Column | 1 x 5 ml | 180201-1x5ML | £94.49 |
Related products | Quantity | Code no. | Price GBP |
SuperSpin Desaltor | 50 | 210101 | £162.29 |
Ready-to-use His Buffer Kit | 2*50ml stock phosphate buffer and 50ml stock imidazole solution | 200105 | £61.64 |
How to order
Pre-packed Ni SepFast Columns