Magnetic IMAC resins
| >>Introduction | >>Product Charactistics | >>Ordering Info | >>How to Order |
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Introduction
Magnetic IMAC (immobilised metal affinity chromatography) resin is typically useful in purifying small quantity low concentration histidine-tagged proteins. For example, when 10 – 50 µl of resin is required for protein purification, it is difficult to handle such small quantities in columns. Magnetic IMAC resins can be easily processed with the aid of a magnet. The liquid / solid separation required in each purification step (e.g. equilibration, binding, washing and elution) is readily achieved with the aid of a magnet (e.g. magnetic stirring bar or other commercial magnetic devices).
Key benefits:
- Tailored base matrix design for batch magnetic purification of proteins
- High binding capacity (could be over 40 mg/ml)
- Simple and convenient operation, no chromatography training required
- Flexible operational conditions
- Low cost
- A choice of three types of immobilised metal ions available
- Very low metal leakage
- Full operation manual (including purification conditions) supplied
Product Characteristics
| Particle size | 50 – 150 µm |
| Base matrix | Cross-linked 6% agarose encapsulating magnetic particles |
| Metal ion capacity | Approx. 12 - 25 µmol / ml resin |
| Protein binding capacity | Depends on the type of proteins and binding conditions; could be over 40 mg/ml resin* |
| Chemical stability** | Stable in0.01M HCl and 1% SDS tested for 30 mins;0.5 M NaOH tested for overnight; |
| pH stability** | 2-14 (<2 h)4-12 (up to one week) |
| Storage | 20% ethanol at 4oC |
*Tested with nickel ion charged; **Tested with the absence of metal ion
Ordering information
| Product | Quantity | Code no. | Price GBP |
| Ni SepFast MAG | 5 ml | 190101 | £97.90 |
| Co SepFast MAG | 5 ml | 190103 | £97.90 |
| Zn SepFast MAG | 5 ml | 190104 | £97.90 |
Note: No magnet is supplied with the above products. Customers need to
source the magnet themselves.
| Related products | Quantity | Code no. | Price GBP |
| SuperSpin Desaltor | 50 | 210101 | £162.29 |
| Ready-to-use His Buffer Kit | 2*50ml stock phosphate buffer and 50ml stock imidazole solution | 200105 | £61.64 |
How to order
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Ni SepFast MAG: Purification of a 6 x His tagged hydrolase from unclarified E.coli. cell lysate in a batch operation
Lane 1: Molecular marker; Lane 2: Unclarified cell lysate; Lane 3: Cell lysate pass through; Lane 4: Wash with binding buffer (20 mM phosphate, 20 mM imidazole and 500 mM NaCl, pH 7.4); Lane 5: Wash with 50 mM imidazole in the binding buffer; Lane 6 and 7: Eluates at 500 mM imidazole in the binding buffer, pH 7.4.
Ni SepFast MAG
