SepFastTM Purifier IEX Media

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Introduction


SepFast Purifier Q, SepFast Purifier DEAE, SepFast Purifier S and SepFast Purifier CM are, respectively, strong anion, weak anion, strong cation and weak cation exchange adsorbents. They are specially designed for intermediate stage or final stage purification of biological molecules in which impure components are chromatographically separated from target molecules.

SepFast Purifier media has a balanced design between loading capacity and separation power of individual components for large-scale biomanufacturing applications. The core advantages are:

  • High sample loading capacity at high flow
  • High separation power at high flow
  • High productivity

The base matrix is made of a composite of polysaccharides that have been highly cross-linked. The media is very stable to most of the chemical conditions experienced in the bioprocessing industry.

 For each type of ion exchange medium, there is a choice of three different base matrices according to pore accessibility of target molecules. The feature and selection guide is listed as follows:

400 serial 500 serial 600 serial
SepFast Purifier
Q-400		 SepFast Purifier
Q-500		 SepFast Purifier
Q-600
SepFast Purifier
DEAE-400		 SepFast Purifier
DEAE-500		 SepFast Purifier
DEAE-600
SepFast Purifier
S-400		 SepFast Purifier
S-500		 SepFast Purifier
S-600
SepFast Purifier
CM-400		 SepFast Purifier
CM-500		 SepFast Purifier
CM-600
The above media is designed to purify smaller components (e.g. M.W. <100K Dalton). The above media is designed to purify antibodies from aggregates and other impurities. The above media is designed to purify large proteins, plasmids or viral particles.

Characteristics of SepFast Purifier IEX Media

 
Purifier Q-400
Purifier Q-500
Purifier Q-600		 Purifier DEAE-400
Purifier DEAE-500
Purifier DEAE-600		 Purifier S-400
Purifier S-500
Purifier S-600		 Purifier CM-400
Purifier CM-500
Purifier CM-600
Matrix Highly cross-linked polysaccharide composites
Functional group Quaternary ammonium strong anion Diethyl-aminoethyl weak anion Sulfo strong cation Carboxy-
methyl weak cation
Total ionic capacity 0.04-0.18 mmol/ml 0.04-0.18 mmol/ml 0.04-0.18 mmol/ml 0.04-0.18 mmol/ml
Particle size 50 – 150 μm
Dynamic binding capacity* >150 mg/ml BSA for Purifier Q-400 >150 mg/ml BSA for Purifier DEAE-400 >60 mg/ml hIgG for Purifier S-500 >120 mg/ml lysozyme for Purifier CM-400
Pressure-flow property** >1000 cm/h for Q-400, DEAE-400, S-400 and CM-400;
>500 cm/h for Q-500, DEAE-500, S-500 and CM-500;
>300 cm/h for Q-600, DEAE-600, S-600 and CM-600
Operational pressure Up to 3 bar
pH stability 2-14 (short term) and 3-12 (long term)
Working temperature 4oC – 30oC
Chemical stability All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol
Total ionic capacity Oxidizing agents, anionic detergents Oxidizing agents, cationic detergents
Storage 20% ethanol 20% ethanol 20% ethanol + 0.2 M sodium acetate 20% ethanol

*Measured at a breakthrough of 10%.
**Measured in a 32 mm ID column at a bed height of 20 cm.

Ordering information

 
SepFast Purifier DEAE Products SepFast Purifier S Products SepFast Purifier CM Products
Purifier Q Products
Description Quantity Code no. Price GBP
SepFast Purifier Q-400 25 ml 460101 £81.00
  100 ml 460102 £226.00
  1 Litre 460103 POR
Disposable SepFast Purifier Q-400 column 5 x 1 ml 460104 £116.00
  1 x 5 ml 460105 £86.00
  1 x 10 ml 460106 £121.00
  1 x 20 ml 460107 £198.00
SepFast Purifier Q-500 25 ml 460201 £81.00
  100 ml 460202 £226.00
  1 Litre 460203 POR
Disposable SepFast Purifier Q-500 column 5 x 1 ml 460204 £116.00
  1 x 5 ml 460205 £86.00
  1 x 10 ml 460206 £121.00
  1 x 20 ml 460207 £198.00
SepFast Purifier Q-600 25 ml 460301 £81.00
  100 ml 460302 £226.00
  1 Litre 460303 POR
Disposable SepFast Purifier Q-600 column 5 x 1 ml 460304 £116.00
  1 x 5 ml 460305 £86.00
  1 x 10 ml 460306 £121.00
  1 x 20 ml 460307 £198.00
SepFast Purifier Q Products SepFast Purifier S Products SepFast Purifier CM Products
Purifier DEAE Products
Description Quantity Code no. Price GBP
SepFast Purifier DEAE-400 25 ml 460401 £81.00
  100 ml 460402 £226.00
  1 Litre 460403 POR
Disposable SepFast Purifier DEAE-400 column 5 x 1 ml 460404 £116.00
  1 x 5 ml 460405 £86.00
  1 x 10 ml 460406 £121.00
  1 x 20 ml 460407 £198.00
SepFast Purifier DEAE-500 25 ml 460501 £81.00
  100 ml 460502 £226.00
  1 Litre 460503 POR
Disposable SepFast Purifier DEAE-500 column 5 x 1 ml 460504 £116.00
  1 x 5 ml 460505 £86.00
  1 x 10 ml 460506 £121.00
  1 x 20 ml 460507 £198.00
SepFast Purifier DEAE-600 25 ml 460601 £81.00
  100 ml 460602 £226.00
  1 Litre 460603 POR
Disposable SepFast Purifier DEAE-600 column 5 x 1 ml 460604 £116.00
  1 x 5 ml 460605 £86.00
  1 x 10 ml 460606 £121.00
  1 x 20 ml 460607 £198.00
SepFast Purifier Q Products SepFast Purifier DEAE Products SepFast Purifier CM Products
Purifier S Products
Description Quantity Code no. Price GBP
SepFast Purifier S-400 25 ml 460701 £81.00
  100 ml 460702 £226.00
  1 Litre 460703 POR
Disposable SepFast Purifier S-400 column 5 x 1 ml 460704 £116.00
  1 x 5 ml 460705 £86.00
  1 x 10 ml 460706 £121.00
  1 x 20 ml 460707 £198.00
SepFast Purifier S-500 25 ml 460801 £81.00
  100 ml 460802 £226.00
  1 Litre 460803 POR
Disposable SepFast Purifier S-500 column 5 x 1 ml 460804 £116.00
  1 x 5 ml 460805 £86.00
  1 x 10 ml 460806 £121.00
  1 x 20 ml 460807 £198.00
SepFast Purifier S-600 25 ml 460901 £81.00
  100 ml 460902 £226.00
  1 Litre 460903 POR
Disposable SepFast Purifier S-600 column 5 x 1 ml 460904 £116.00
  1 x 5 ml 460905 £86.00
  1 x 10 ml 460906 £121.00
  1 x 20 ml 460907 £198.00
SepFast Purifier Q Products SepFast Purifier DEAE Products SepFast Purifier S Products
Purifier CM Products
Description Quantity Code no. Price GBP
SepFast Purifier CM-400 25 ml 461001 £81.00
  100 ml 461002 £226.00
  1 Litre 461003 POR
Disposable SepFast Purifier CM-400 column 5 x 1 ml 461004 £116.00
  1 x 5 ml 461005 £86.00
  1 x 10 ml 461006 £121.00
  1 x 20 ml 461007 £198.00
SepFast Purifier CM-500 25 ml 461101 £81.00
  100 ml 461102 £226.00
  1 Litre 461103 POR
Disposable SepFast Purifier CM-500 column 5 x 1 ml 461104 £116.00
  1 x 5 ml 461105 £86.00
  1 x 10 ml 461106 £121.00
  1 x 20 ml 461107 £198.00
SepFast Purifier CM-600 25 ml 461201 £81.00
  100 ml 461202 £226.00
  1 Litre 461203 POR
Disposable SepFast Purifier CM-600 column 5 x 1 ml 461204 £116.00
  1 x 5 ml 461205 £86.00
  1 x 10 ml 461206 £121.00
  1 x 20 ml 461207 £198.00


>>Click here for SepFast Purifier Q Products

>>Click here for SepFast Purifier DEAE Products

>>Click here for SepFast Purifier S Products

>>Click here for SepFast Purifier CM Products

How to order

Products

Data Sheet

Data sheet