Protein A SuperSpin
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Introduction
Protein A SuperSpin is supplied for fast small-scale purification of immunoglobulins from various species (e.g. serum, ascites and mammalian cell culture broth etc). Protein A has been well proven a powerful affinity ligand which shows very high specificity to immunoglobulins from mammalian hosts.
Each Protein A SuperSpin tube accommodates 50 µl of Protein A SepFast particles. The affinity resin is specifically designed to provide large pore size with small particle size (i.e. 30 – 100 µm). Thus large antibody molecules can access the affinity ligand rapidly with excellent mass transfer rate. As a result, half milligram quantities of antibody can be easily purified within 20 minutes at low cost. No special expensive chromatography instrument or column is required.
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Protein A SuperSpin is particularly useful for the following applications:
- The quantity of antibody to be purified is small (less than half a milligram)
- On-line detection of antibody expression level
- High throughput purification of antibodies
- High throughput screening of antibodies
- Key benefits:
- Rapid antibody capture and recovery
- High antibody binding capacity (half milligram)
- High throughput format (24 samples can be processed in parallel)
- Low cost
- Easy to use and no expensive equipment required
Characteristics of Protein A SuperSpin
Tube material | Polypropylene |
Resin | Protein A SepFast |
Particle size | 30 – 100 µm |
Packed volume | 50 µl |
Protein binding capacity | Depends on the type of proteins and binding conditions; up to 500 µg |
Chemical compatibility | The resin maintains the binding capability after exposing to 3M NaSCN, 6M GuHCl or 8M urea over a few hours |
Storage condition | 4oC – 30oC |
Ordering information
Product | Quantity | Code no. | Price GBP |
Protein A SuperSpin | 50 | 280301 | £263.50 |
Related products | Quantity | Code no. | Price GBP |
SuperSpin Desaltor | 50 | 210101 | £162.29 |
How to order
Protein A SuperSpin