Protein A SuperSpin
Introduction
Protein A SuperSpin is supplied for fast small-scale purification of
immunoglobulins from various species (e.g. serum, ascites and mammalian
cell culture broth etc). Protein A has been well proven a powerful affinity
ligand which shows very high specificity to immunoglobulins from mammalian hosts.
Each Protein A SuperSpin tube accommodates 50 µl of Protein A SepFast particles.
The affinity resin is specifically designed to provide large pore size with
small particle size (i.e. 30 – 100 µm). So large antibody molecules can access
the affinity ligand rapidly with excellent mass transfer rate. As a result, half
milligram quantity of antibody can be easily purified within 20 minutes with
inexpensive cost. No special expensive chromatography instrument and column is
required.
Protein A SuperSpin is particularly useful for the following applications:
- The quantity of antibody to be purified is small (less than half milligram)
- On-line detection of antibody expression level
- High throughput purification of antibodies
- High throughput screening of antibodies
Key benefits:
- Rapid antibody capture and recovery
- High antibody binding capacity (half milligram)
- High throughput format (24 samples can be processed in parallel)
- Low cost
- Easy to use and no expensive equipment required
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Characteristics of Protein A SuperSpin
| Tube material |
Polypropylene |
| Resin |
Protein A SepFast |
| Particle size |
30 – 100 µm |
| Packed volume |
50 µl |
| Protein binding capacity |
Depends on the type of proteins and binding conditions; up to 500
µg |
| Chemical compatibility |
The resin maintains the binding capability after exposing to 3M NaSCN, 6M
GuHCl or 8M urea over a few hours |
| Storage condition |
4oC – 30oC |
Ordering information
Ordering information
| Related products |
Quantity |
Code no. |
Price GBP |
| SuperSpin Desaltor |
50 |
210101 |
£121.00 |