Protein A SepFastTM Resins and Columns
| >>Introduction | >>Product Charactistics | >>Ordering Info | >>How to Order |
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Introduction
Protein A SepFast is an affinity chromatography medium for the purification of immunoglobulins. Its purification power has been well documented in various antibody purification applications, such as isolation and purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.
Protein A is immobilised to highly porous and highly cross-linked agarose
base matrix. Agarose has long been used for chromatographic separations due to
its excellent hydrophilic and low non-specific-binding nature. The particle has
open pore structure with excellent mass transfer property to large protein
molecules. The medium shows high mechanical rigidity. So it can be operated at
moderate to high flow velocities with moderate pressure drop.
BioToolomics offers both loose medium and pre-packed ready-to-use disposable
columns.
Characteristics of Protein A SepFast resins and columns
| Matrix | Highly cross-linked agarose |
| Column material | Polypropylene / acrylic |
| Operating pressure | up to 3 bar |
| Binding capacity | >30 mg human IgG/ml |
| Chemical compatibility | The commonly used reagents for antibody purifications |
| Storage condition | 4oC – 8oC |
*Tested with nickel ion charged
Ordering information
| Product | Quantity | Code no. | Price GBP |
| Protein A SepFast | 1 ml | 230103 | £ 50.00 |
| Protein A SepFast | 5 ml | 230104 | £152.00 |
| Protein A SepFast | 25 ml | 230105 | £685.00 |
| Protein A SepFast Column | 1 x 1 ml | 230107 | £ 59.00 |
| Protein A SepFast Column | 1 x 5 ml | 230108 | £181.00 |
How to order
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Protein A SepFast Resins & Columns
