MabPolish^® (Type I, Type II)

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Introduction

MabPolish is special mixed-mode chromatography media having strong binding to a broad range of host cell proteins and other impurities but little binding to antibodies (typically >90% yield). It is designed for process scale production of antibodies that requires regulatory support files.

	Application Guide
MabPolishTM Type I	Anion mixed-mode resin with very mild hydrophobicity. It can remove high level of HCPs at pH 4 to 5 and salt concentration up to 0.15 M.
MabPolishTM Type II	Anion mixed-mode resin with hydrophobicity stronger than Type I. It can remove more hydrophobic species at pH 4 to 5 and salt concentration up to 0.15 M.

MabPolish can be used in flow-through mode to remove impurities from monoclonal antibodies or other antibody materials, at various stages of the whole antibody purification process. Examples of the possible application scenario could be:

• Direct removal of impurities from crude clarified antibody materials
• After an antibody is first purified by affinity chromatography media (e.g. Protein A, Protein G or Protein L etc);
• After an antibody is first purified by ion-exchangers;
• After an antibody is first processed through precipitation

The base matrix is made of beaded agarose that has been highly cross-linked. The media is stable in most chemical conditions experienced in the bioprocessing industry.

Characteristics of MabPolish

Matrix	Spherical beads of cross-linked agarose
Particle size	50 – 150 µm
Functional group	Mixed mode ligands involving multi interaction mechanisms
Operational pressure	Up to 3 bar
pH stability	2-14 (short term) and 3-12 (long term)
Working temperature	+4oC to +30oC
Chemical stability	All commonly used buffers
Avoid	Oxidizing agents, detergents

Ordering information

Ordering information

Product	Quantity	Code no.	Price GBP
MabPolish Type I	25 ml	270201-25ML	£156.45
	100 ml	270201-100ML	POR
	1 litre	270201-1L	POR
Pre-packed column	5 x 1 ml	270201-5x1ML	£149.00
	1 x 10 ml	270201-1x10ML	£196.35
MabPolish Type II	25 ml	270202-25ML	£156.45
	100 ml	270202-100ML	POR
	1 litre	270202-1L	POR
Pre-packed column	5 x 1 ml	270202-5x1ML	£149.00
	1 x 10 ml	270202-1x10ML	£196.35
MabPolish Selection Kit	6 x 1 ml (1 ml each of MabPolish Type I, Type II, MabPolish DUO 150A, 150C, 400A, 400C)	270000-6x1ML	£198.45

*Other column size available on request.

How to order